PXD004103-1

PXD004103 is an original dataset announced via ProteomeXchange.

Title	Comparative interactomes of VRK1 and VRK3: implication in cell cycle, chromatin assembly, RNA processing and DNA repair
Description	Vaccinia related kinase (VRK) family is a ser/threonine kinase. VRK1 and VRK3 are localized in the same organelle, mainly in the nucleus. Comparative interactomes of VRK1 and VRK3 were not performed yet. We identified over 200 unreported VRK1/3-interacting proteins by proteomics-based analyses using the complementary mass spectrometry approach. Among those proteins, nuclear and cytoplasmic proteins were mostly enriched reflecting nuclear and cytoplasmic localization of VRK1/3. The interaction networks of interactome indicated biological processes associated with cell cycle, DNA repair, chromatin assembly and RNA processing. Interactions and co-localization of NCL, PARP1, HSP70, XRCC5, p53, NPM1, BAF1, cyclinB1 and ALYREF with VRK1/3 were confirmed by co-immunoprecipitation and immunofluorescent staining. Overall, this study represents a valuable resource for investigating VRK1 and VRK3 functions in health and disease, encompassing emerging themes of VRK1/3 regulation in cell cycle, mRNA processing, chromatin assembly and DNA repair.
HostingRepository	PRIDE
AnnounceDate	2018-10-26
AnnouncementXML	Submission_2018-10-26_06:25:19.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Dae-Kyum Kim
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	No PTMs are included in the dataset
Instrument	LTQ Orbitrap Velos

Revision	Datetime	Status	ChangeLog Entry
0	2016-05-05 01:31:10	ID requested
⏵ 1	2018-10-26 06:25:21	announced
2	2024-10-22 04:29:23	announced	2024-10-22: Updated project metadata.

Lee N, Kim DK, Han SH, Ryu HG, Park SJ, Kim KT, Choi KY, Comparative Interactomes of VRK1 and VRK3 with Their Distinct Roles in the Cell Cycle of Liver Cancer. Mol Cells, 40(9):621-631(2017) [pubmed]

curator keyword: Biological

submitter keyword: VRK1, VRK3, interactome

Kwan Yong Choi
contact affiliation	Department of Life Science and Department of Integrative Biosciences & Biotechnology, Pohang University of Science and Technology, 77 Cheongam-Ro, Nam-Gu, Pohang, Kyungbuk, 790-784, Republic of Korea
contact email	kchoi@postech.ac.kr
lab head
Dae-Kyum Kim
contact affiliation	Department of Life Science
contact email	sses162@postech.ac.kr
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2018/10/PXD004103

PRIDE project URI

• PRIDE
  1. PXD004103
     1. Label: PRIDE project
     2. Name: Comparative interactomes of VRK1 and VRK3: implication in cell cycle, chromatin assembly, RNA processing and DNA repair