PXD004062 is an
original dataset announced via ProteomeXchange.
Dataset Summary
Title | Changes in the membrane proteome of exosomes released from human macrophages after infection with Mycobacterium tuberculosis. |
Description | One of the greatest obstacles to eliminating TB is the lack of predictive and diagnostic biomarkers. Exosomes are a promising alternative source of biomarkers for TB since they can carry mycobacterial antigens. We hypothesize that exosomes from Mtb-infected macrophages exhibit a characteristic selection of human protein that can be evaluated as TB biomarkers. We looked for the localization—exosomal membrane or lumen—of the differentially abundant proteins. Exosomes were obtained from THP-1-derived macrophages (Mtb infected and uninfected controls). The exosome population was validated by nanoparticle tracking analysis and western blot. The protein composition of exosomes was evaluated by tandem mass spectrometry. Differences in protein composition and abundances between exosomes from infected and control cells were evaluated by t-test the proteomics findings were confirmed by western blot. Using a biotinylation strategy we verified the protein localization. Forty-seven proteins were significantly more abundant in exosomes from Mtb-infected cells, 66% were predicted to be membrane associated. The biotinylation pattern confirmed the membrane-association of some of these proteins. |
HostingRepository | PRIDE |
AnnounceDate | 2016-12-20 |
AnnouncementXML | Submission_2016-12-20_14:13:32.xml |
DigitalObjectIdentifier | https://dx.doi.org/10.6019/PXD004062 |
ReviewLevel | Peer-reviewed dataset |
DatasetOrigin | Original dataset |
RepositorySupport | Supported dataset by repository |
PrimarySubmitter | Gustavo Diaz |
SpeciesList | scientific name: Homo sapiens (Human); NCBI TaxID: 9606; |
ModificationList | No PTMs are included in the dataset |
Instrument | LTQ Orbitrap Velos |
Dataset History
Revision | Datetime | Status | ChangeLog Entry |
0 | 2016-04-27 02:05:08 | ID requested | |
⏵ 1 | 2016-12-20 14:13:33 | announced | |
2 | 2017-10-24 03:10:15 | announced | Updated project metadata. |
Publication List
Diaz G, Wolfe LM, Kruh-Garcia NA, Dobos KM, Changes in the Membrane-Associated Proteins of Exosomes Released from Human Macrophages after Mycobacterium tuberculosis Infection. Sci Rep, 6():37975(2016) [pubmed] |
Keyword List
curator keyword: Biological, Biomedical |
submitter keyword: Mycobacterium tuberculosis |
exosomes |
membrane proteins |
macrophage |
Contact List
Karen Dobos |
contact affiliation | Director, Research Integrity and Compliance Review Office (http://ricro.colostate.edu/) Associate Professor, Mycobacteria Research Laboratories Dept of Microbiology, Immunology,& Pathology (http://www.cvmbs.colostate.edu/DirectorySearch/Search/MemberProfile/CVMBS/3607) Colorado State University |
contact email | karen.dobos@colostate.edu |
lab head | |
Gustavo Diaz |
contact affiliation | Colorado State University |
contact email | diaz.gustavo2011@gmail.com |
dataset submitter | |
Full Dataset Link List
Dataset FTP location
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PRIDE project URI |
Repository Record List
[ + ]
[ - ]
- PRIDE
- PXD004062
- Label: PRIDE project
- Name: Changes in the membrane proteome of exosomes released from human macrophages after infection with Mycobacterium tuberculosis.