PXD004028-1
PXD004028 is an original dataset announced via ProteomeXchange.
Dataset Summary
| Title | System Biology approach to protein stability regulation in yeast |
| Description | Cellular protein levels depend upon their synthesis and degradation rates (i.e protein turnover). Characterizing protein turnover is thus essential for determining cell response to different stimuli but the main determinants of this process are poorly understood. We comprehensively addressed this by applying a systems biology approach integrating protein stability with physical, biological and genetic information in yeast. The analysis of >3000 protein abundances and half-lives by pulsed SILAC support a pro-degradative design (i.e. higher lysine density, accumulation of ubiquitylation sites in C-terminal and increase of proteolytic degradation signs) for unstable proteins. However, functional characteristics of proteins, such as localization, connectivity and activity, are also a major determinants of protein stability. We also provide evidence of a synchronization between protein and mRNA stabilities in exponentially growing yeast. Finally, we showed that stress induced changes in protein and transcript abundance are directly correlated to changes in protein turnover.This study establish the bottom line for future studies aimed at deciphering protein turnover regulation |
| HostingRepository | MassIVE |
| AnnounceDate | 2017-09-25 |
| AnnouncementXML | Submission_2017-09-25_08:34:15.xml |
| DigitalObjectIdentifier | |
| ReviewLevel | Non peer-reviewed dataset |
| DatasetOrigin | Original dataset |
| RepositorySupport | Unsupported dataset by repository |
| PrimarySubmitter | Miguel Martin Perez |
| SpeciesList | scientific name: Saccharomyces cerevisiae; common name: baker's yeast; NCBI TaxID: 4932; |
| ModificationList | No PTMs are included in the dataset |
| Instrument | Q Exactive |
Dataset History
| Revision | Datetime | Status | ChangeLog Entry |
|---|---|---|---|
| 0 | 2016-04-21 08:19:32 | ID requested | |
| ⏵ 1 | 2017-09-25 08:34:16 | announced |
Publication List
| no publication |
Keyword List
| submitter keyword: turnover, half-life, osmotic stress |
Contact List
| Judit Vill�n | |
|---|---|
| lab head | |
| Miguel Martin Perez | |
| contact affiliation | University of Washington |
| contact email | mmarper@uw.edu |
| dataset submitter | |
Full Dataset Link List
| MassIVE dataset URI |
| Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://massive.ucsd.edu/MSV000079673 |
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