PXD004009 is an
original dataset announced via ProteomeXchange.
Dataset Summary
Title | A whole proteome inventory of background photocrosslinker binding |
Description | Affinity based protein profiling (AfBPP) is a widely applied methodology for target identification of bioactive molecules. Photocrosslinkers such as benzophenone, diazirine and aryl azide irreversibly link the molecule of interest to its dedicated target protein upon irradiation with UV light. Despite their prevalent application in chemical biology little is known about photolinker-specific off-targets affecting the reliability of results. Here we investigate background protein labelling in intact human cell lines via gel-free quantitative proteomics. Characteristic off-targets were identified for each photo-reactive group and compiled in a comprehensive inventory (photome). In a proof of principle study, H8, an established inhibitor of protein kinase A (PKA), was equipped with a diazirine moiety and revealed, by alignment with the photo-background, unprecedented insight into its in situ proteome targets. Taken together, our findings provide an experimental guideline to surmount photoprobe ambiguity and focus results on biologically relevant binders. |
HostingRepository | PRIDE |
AnnounceDate | 2016-12-06 |
AnnouncementXML | Submission_2016-12-06_00:43:04.xml |
DigitalObjectIdentifier | |
ReviewLevel | Peer-reviewed dataset |
DatasetOrigin | Original dataset |
RepositorySupport | Unsupported dataset by repository |
PrimarySubmitter | Matthias Stahl |
SpeciesList | scientific name: Homo sapiens (Human); NCBI TaxID: 9606; |
ModificationList | monohydroxylated residue; acetylated residue; iodoacetamide derivatized residue |
Instrument | Orbitrap Fusion |
Dataset History
Revision | Datetime | Status | ChangeLog Entry |
0 | 2016-04-19 02:19:45 | ID requested | |
⏵ 1 | 2016-12-06 00:43:04 | announced | |
2 | 2023-11-14 06:38:27 | announced | 2023-11-14: Updated project metadata. |
Publication List
Keyword List
curator keyword: Technical |
submitter keyword: photocrosslinker, target identification, affinity-based protein profiling |
Contact List
Stephan Axel Sieber |
contact affiliation | Lehrstuhl Organische Chemie II, Department of Chemistry, Technische Universität München, Lichtenbergstraße 4, 85747 Garching b. München |
contact email | stephan.sieber@tum.de |
lab head | |
Matthias Stahl |
contact affiliation | Technische Universität München |
contact email | matthias.stahl@tum.de |
dataset submitter | |
Full Dataset Link List
Dataset FTP location
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PRIDE project URI |
Repository Record List
[ + ]
[ - ]
- PRIDE
- PXD004009
- Label: PRIDE project
- Name: A whole proteome inventory of background photocrosslinker binding