PXD003982-1

PXD003982 is an original dataset announced via ProteomeXchange.

Title	Profiling carbonylated proteins in heart and skeletal muscle mitochondria from trained and untrained mice
Description	Understanding the relationship between physical exercise, reactive oxygen species and skeletal muscle modification is important in order to better identify the benefits or the damages that appropriate or inappropriate exercise can induce. Heart and skeletal muscles have a high density of mitochondria with robust energetic demands and mitochondria plasticity has an important role in both cardiovascular system and skeletal muscle responses. The aim of this study was to investigate the influence of regular physical activity on oxidation profiles of mitochondrial proteins from heart and tibialis anterior muscles. To this end, we used mouse as animal model. Mice were divided in two groups: untrained and regularly trained. The carbonylated protein pattern was studied by two-dimensional gel electrophoresis followed by Western Blot with anti-dinitrophenyl hydrazone antibodies. Mass spectrometry analysis allowed the identifications of several different protein oxidation sites including methionine, cysteine, proline and leucine residues. A large number of oxidized protein were found in both untrained and trained animals. Moreover, mitochondria from skeletal muscles and heart showed almost the same carbonylation pattern. Interestingly, exercise training seems to increase carbonylation level mostly of mitochondrial protein from skeletal muscle.
HostingRepository	PRIDE
AnnounceDate	2022-02-24
AnnouncementXML	Submission_2022-02-24_06:55:23.191.xml
DigitalObjectIdentifier	https://dx.doi.org/10.6019/PXD003982
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Supported dataset by repository
PrimarySubmitter	Andrea Carpentieri
SpeciesList	scientific name: Rattus norvegicus (Rat); NCBI TaxID: 10116;
ModificationList	monomethylated residue; didehydrogenated residue; amidated residue; monohydroxylated residue; deaminated residue; glutamyl semialdehyde (Arg); L-cysteic acid (L-cysteine sulfonic acid); nitrated L-tyrosine; deamidated residue; iodoacetamide derivatized residue
Instrument	LTQ Orbitrap

Revision	Datetime	Status	ChangeLog Entry
0	2016-04-18 05:54:49	ID requested
⏵ 1	2022-02-24 06:55:23	announced
2	2023-11-14 08:49:26	announced	2023-11-14: Updated project metadata.

10.1021/ACS.JPROTEOME.6B00475;

curator keyword: Biological, Biomedical

submitter keyword: two dimensional gel electrophoresis , tandem mass spectrometry, Carbonylation, Orbi Trap

Andrea Carpentieri
contact affiliation	Università di Napoli Federico II
contact email	acarpent@unina.it
lab head
Andrea Carpentieri
contact affiliation	Chemical Sciences
contact email	acarpent@unina.it
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD003982
     1. Label: PRIDE project
     2. Name: Profiling carbonylated proteins in heart and skeletal muscle mitochondria from trained and untrained mice