PXD003961-2

PXD003961 is an original dataset announced via ProteomeXchange.

Title	Sec62 regulates ER-phagy during recovery from ER stress
Description	Perturbing ER homeostasis activates stress programs collectively called the unfolded protein response (UPR). The UPR enhances production of ER-resident chaperones and enzymes to reduce the burden of misfolded proteins. Upon resolution of ER stress, excess ER material is removed by ill-defined, selective autophagic programs. Here, using biochemical and cell-based essays, we identify a novel ER-resident autophagy receptor, Sec62, which is activated during recovery from ER stress to selectively deliver ER fragments to the autolysosomal system for clearance. Quantitative mass spectrometry analyses of autolysosomal fractions upon selective inactivation of the Sec62-regulated pathway inform on the selectivity of Sec62-regulated ER-phagy.
HostingRepository	PRIDE
AnnounceDate	2016-10-11
AnnouncementXML	Submission_2016-10-24_00:59:18.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Manfredo Quadroni
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	monohydroxylated residue; acetylated residue
Instrument	Q Exactive

Revision	Datetime	Status	ChangeLog Entry
0	2016-04-13 04:30:15	ID requested
1	2016-10-11 01:02:29	announced
⏵ 2	2016-10-24 00:59:19	announced	Updated publication reference for PubMed record(s): 27749824.
3	2024-10-22 04:05:07	announced	2024-10-22: Updated project metadata.

Fumagalli F, Noack J, Bergmann TJ, Cebollero E, Pisoni GB, Fasana E, Fregno I, Galli C, Loi M, Sold, à T, D'Antuono R, Raimondi A, Jung M, Melnyk A, Schorr S, Schreiber A, Simonelli L, Varani L, Wilson-Zbinden C, Zerbe O, Hofmann K, Peter M, Quadroni M, Zimmermann R, Molinari M, Translocon component Sec62 acts in endoplasmic reticulum turnover during stress recovery. Nat Cell Biol, 18(11):1173-1184(2016) [pubmed]

curator keyword: Biological

submitter keyword: ER stress, autophagy, ERAD, label-free quantitation, endoplasmic reticulum

Maurizio Molinari
contact affiliation	Institute for Research in Biomedicine (IRB) Via Vela 6 CH-6500 Bellinzona Switzerland
contact email	maurizio.molinari@irb.usi.ch
lab head
Manfredo Quadroni
contact affiliation	University of Lausanne
contact email	manfredo.quadroni@unil.ch
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2016/10/PXD003961

PRIDE project URI

• PRIDE
  1. PXD003961
     1. Label: PRIDE project
     2. Name: Sec62 regulates ER-phagy during recovery from ER stress