PXD003920 is an
original dataset announced via ProteomeXchange.
Dataset Summary
Title | Shotgun Proteomics of Tomato Fruits: Evaluation and Optimization of Sample Preparation Methods and Mass Spectrometric Parameters |
Description | Optimization of sample preparation protocols and mass spectrometric parameters is essential for adequate proteome coverage. Though several protocols are available for 2D electrophoresis-based proteome profiling, very few protocols exist for sample preparation for shotgun proteomics and optimization of mass spectrometric parameters using plant tissues. Here, we report an optimized protocol for shotgun proteomics of tomato fruit, which is a recalcitrant tissue comprising of low concentration of proteins and high percentage of sugars and secondary metabolites |
HostingRepository | PRIDE |
AnnounceDate | 2016-07-26 |
AnnouncementXML | Submission_2016-07-26_03:42:53.xml |
DigitalObjectIdentifier | |
ReviewLevel | Peer-reviewed dataset |
DatasetOrigin | Original dataset |
RepositorySupport | Unsupported dataset by repository |
PrimarySubmitter | Yellamaraju Sreelakshmi |
SpeciesList | scientific name: Solanum lycopersicum; NCBI TaxID: 4081; |
ModificationList | monohydroxylated residue; deamidated residue; iodoacetamide derivatized residue |
Instrument | LTQ Orbitrap Velos |
Dataset History
Revision | Datetime | Status | ChangeLog Entry |
0 | 2016-04-06 07:34:51 | ID requested | |
1 | 2016-06-30 03:51:00 | announced | |
⏵ 2 | 2016-07-26 03:42:54 | announced | Updated publication reference for PubMed record(s): 27446192. |
Publication List
Kilambi HV, Manda K, Sanivarapu H, Maurya VK, Sharma R, Sreelakshmi Y, Shotgun Proteomics of Tomato Fruits: Evaluation, Optimization and Validation of Sample Preparation Methods and Mass Spectrometric Parameters. Front Plant Sci, 7():969(2016) [pubmed] |
Keyword List
curator keyword: Biological |
submitter keyword: Tomato fruit, shotgun proteomics, sample preparation, fractionation, proteome coverage, label free quantification, spectral counting |
Contact List
Yellamaraju Sreelakshmi |
contact affiliation | Assistant Professor, RTGR (Tomato building), Department of Plant Sciences, School of Life Sciences, University of Hyderabad, Gachibowli, Hyderabad, 500046. |
contact email | syellamaraju@gmail.com |
lab head | |
Yellamaraju Sreelakshmi |
contact affiliation | assistant Professor at University of Hyderabad |
contact email | syellamaraju@gmail.com |
dataset submitter | |
Full Dataset Link List
Dataset FTP location
NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2016/06/PXD003920 |
PRIDE project URI |
Repository Record List
[ + ]
[ - ]
- PRIDE
- PXD003920
- Label: PRIDE project
- Name: Shotgun Proteomics of Tomato Fruits: Evaluation and Optimization of Sample Preparation Methods and Mass Spectrometric Parameters