PXD003884 is an
original dataset announced via ProteomeXchange.
Dataset Summary
Title | Peptidomics of Acanthoscurria gomesiana spider venom reveals new toxins with potential antimicrobial activity |
Description | Acanthoscurria gomesiana is a Brazilian spider from the Theraphosidae family inhabiting regions of Southeastern Brazil. Potent antimicrobial peptides as gomesin and acanthoscurrin have been discovered from the spider hemolymph in previous works. Spider venoms are also recognized as sources of biologically active peptides, however the venom peptidome of A. gomesiana remained unexplored to date. In this work, a MS-based workflow was applied to the investigation of the spider venom peptidome. Data-independent and data-dependent LC-MS/MS acquisitions of intact peptides and of peptides submitted to multiple enzyme digestions, followed by automated chromatographic alignment, de novo analysis, database and homology searches with manual validations showed that the venom is composed by less than 165 features, with masses ranging from 0.4-15.8 kDa. A total of 135 peptides from 17 proteins were identified, including three new mature peptides: U1-TRTX-Agm1a, U1-TRTX-Agm2a and U1-TRTX-Agm3a, containing 3, 4 and 3 disulfide bonds, respectively. U1-TRTX-Agm1a differed by only one amino acid from U1-TRTX-Ap1a from A. paulensis and U1-TRTX-Agm2a was derived from the genicutoxin-D1 precursor from A. geniculata. These toxins have potential applications as antimicrobial agents, as the peptide fraction of A. gomesiana showed activity against Escherichia coli strains. |
HostingRepository | PRIDE |
AnnounceDate | 2016-08-02 |
AnnouncementXML | Submission_2016-08-02_06:24:44.xml |
DigitalObjectIdentifier | |
ReviewLevel | Peer-reviewed dataset |
DatasetOrigin | Original dataset |
RepositorySupport | Unsupported dataset by repository |
PrimarySubmitter | Alexandre Keiji Tashima |
SpeciesList | scientific name: Acanthoscurria gomesiana (Tarantula spider) (Phormictopus pheopygus); NCBI TaxID: 115339; |
ModificationList | monohydroxylated residue; acetylated residue; iodoacetamide derivatized residue |
Instrument | LTQ Orbitrap Velos |
Dataset History
Revision | Datetime | Status | ChangeLog Entry |
0 | 2016-03-31 01:10:15 | ID requested | |
⏵ 1 | 2016-08-02 06:24:45 | announced | |
2 | 2024-10-22 04:03:49 | announced | 2024-10-22: Updated project metadata. |
Publication List
Abreu TF, Sumitomo BN, Nishiyama MY, Oliveira UC, Souza GH, Kitano ES, Zelanis A, Serrano SM, Junqueira-de-Azevedo I, Silva PI, Tashima AK, Peptidomics of Acanthoscurria gomesiana spider venom reveals new toxins with potential antimicrobial activity. J Proteomics, 151():232-242(2017) [pubmed] |
Keyword List
curator keyword: Biological, Biomedical |
submitter keyword: Acanthoscurria gomesiana, peptidomics, antimicrobial peptide, theraphotoxin. |
Contact List
Alexandre Keiji Tashima |
contact affiliation | Departamento de Bioquimica, Escola Paulista de Medicina, Universidade Federal de Sao Paulo, SP, Brazil |
contact email | aktashima@unifesp.br |
lab head | |
Alexandre Keiji Tashima |
contact affiliation | Universidade Federal de Sao Paulo |
contact email | aktashima@unifesp.br |
dataset submitter | |
Full Dataset Link List
Dataset FTP location
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PRIDE project URI |
Repository Record List
[ + ]
[ - ]
- PRIDE
- PXD003884
- Label: PRIDE project
- Name: Peptidomics of Acanthoscurria gomesiana spider venom reveals new toxins with potential antimicrobial activity