PXD003882-1

PXD003882 is an original dataset announced via ProteomeXchange.

Title	Drosophila mRNA Interactome
Description	The maternal-to-zygotic transition (MZT) is a process that occurs in animal embryos at the earliest developmental stages, during which maternally deposited mRNAs and other molecules are degraded and replaced by products of the zygotic genome. The zygotic genome is not activated immediately upon fertilization, therefore post-transcriptional mechanisms control the first steps of development in the early, pre-MZT embryo. To perform unbiased organism-wide identification of Drosophila RNA binding proteins (RPBs), crucial players of post-transcriptional control, we applied the recently developed RNA interactome capture method, which involves cross-linking of RNAs and their direct protein partners by UV light, purification of RNA under stringent conditions and identification of proteins by mass spectrometry. Our analysis yielded 523 high confidence RBP hits, half of which were not previously reported to bind RNA. Our comparison of the RNA interactomes of pre- and post-MZT embryos reveals a highly dynamic behavior of the RNA-bound proteome during early development, and suggests active regulation of RNA binding of some RBPs. This resource provides the first evidence of RNA binding for hundreds of Drosophila proteins, and opens new avenues for study of molecular mechanisms of early development.
HostingRepository	PRIDE
AnnounceDate	2016-07-11
AnnouncementXML	Submission_2016-07-11_06:16:53.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Christian Frese
SpeciesList	scientific name: Drosophila melanogaster (Fruit fly); NCBI TaxID: 7227;
ModificationList	TMT6plex-126 reporter+balance reagent acylated residue; monohydroxylated residue; iodoacetamide derivatized residue
Instrument	LTQ Orbitrap Velos

Revision	Datetime	Status	ChangeLog Entry
0	2016-03-31 00:32:29	ID requested
⏵ 1	2016-07-11 06:16:54	announced
2	2024-10-22 04:03:56	announced	2024-10-22: Updated project metadata.

Sysoev VO, Fischer B, Frese CK, Gupta I, Krijgsveld J, Hentze MW, Castello A, Ephrussi A, Global changes of the RNA-bound proteome during the maternal-to-zygotic transition in Drosophila. Nat Commun, 7():12128(2016) [pubmed]

curator keyword: Biological

submitter keyword: Drosophila development, RNA-binding proteins, TMT-MS3

Jeroen Krijgsveld
contact affiliation	DKFZ German Cancer Research Center Proteomics of Stem Cells and Cancer (B230) Im Neuenheimer Feld 581 69120 Heidelberg, Germany
contact email	j.krijgsveld@dkfz-heidelberg.de
lab head
Christian Frese
contact affiliation	DKFZ German Cancer Research Center
contact email	frese@embl.de
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2016/07/PXD003882

PRIDE project URI

• PRIDE
  1. PXD003882
     1. Label: PRIDE project
     2. Name: Drosophila mRNA Interactome