PXD003861-1
PXD003861 is an original dataset announced via ProteomeXchange.
Dataset Summary
Title | MudPIT analysis of HIV-1 Vpr-asssociated proteins from the permissive CEM.SS human T4-lymphoblastoid cell line |
Description | CEM.SS-iH1.Vpr cells expressing a tandem HA-FLAG- epitope-tagged HIV-1 NL4-3 Vpr (hfa-H.Vpr) under control of the Lenti-X Tet-On 3G Inducible Expression System (Clontech), and control CEM.SS cells (3x105 cells/ml, 4 liters), were induced with doxycycline (0.1ug/ml) for 9 hours. Whole cell extracts were prepared and tandem affinity purifications of Vpr and its associated proteins, from 5 grams of wet cell mass, were performed as described (Hrecka K, et al. Nature 2011; 474(7353):658-661). The Vpr-protein complexes and negative controls purified from T cells were digested with endoproteinase LysC followed by trypsin and the resulting peptide mixtures were analyzed by Multidimensional Protein Identification Technology (MudPIT) on an LTQ ion trap mass spectrometer as described (Florens L, Washburn MP. Methods Mol Biol 2006;328:159-75). |
HostingRepository | MassIVE |
AnnounceDate | 2016-06-22 |
AnnouncementXML | Submission_2016-06-22_13:04:01.xml |
DigitalObjectIdentifier | |
ReviewLevel | Non peer-reviewed dataset |
DatasetOrigin | Original dataset |
RepositorySupport | Unsupported dataset by repository |
PrimarySubmitter | Laurence Florens |
SpeciesList | scientific name: Homo sapiens; common name: human; NCBI TaxID: 9606; scientific name: HIV-1 vector pNL4-3; NCBI TaxID: 151458; |
ModificationList | S-carboxamidomethyl-L-cysteine |
Instrument | LTQ |
Dataset History
Revision | Datetime | Status | ChangeLog Entry |
---|---|---|---|
0 | 2016-03-24 11:22:07 | ID requested | |
⏵ 1 | 2016-06-22 13:04:02 | announced |
Publication List
Dataset with its publication pending |
Keyword List
submitter keyword: HIV accessory virulence protein Vpr, affinity purification, T cells |
Contact List
Laurence FLorens | |
---|---|
lab head | |
Laurence Florens | |
contact affiliation | Stowers Institute for Medical Research |
contact email | laf@stowers.org |
dataset submitter |
Full Dataset Link List
MassIVE dataset URI |
Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://massive.ucsd.edu/MSV000079605 |