PXD003856-3

PXD003856 is an original dataset announced via ProteomeXchange.

Title	¬¬ZMYND8 co-localizes with NuRD on target genes and regulates poly-ADP-ribose dependent recruitment of GATAD2A/NuRD to sites of DNA damage
Description	The NuRD complex is generally thought to repress transcription at both hyper- and hypomethylated regions in the genome. In addition, the complex is involved in the DNA damage response. Here, we show that ZMYND8 bridges NuRD to a number of putative DNA-binding zinc finger proteins. The ZMYND8 MYND domain directly interacts with PPPLΦ motifs in the NuRD subunit GATAD2A. Furthermore, GATAD2A and GATAD2B exclusively form homodimers and they thus define mutually exclusive NuRD subcomplexes. ZMYND8 and MBD3 share a large number of genome-wide binding sites, mostly active promoters and enhancers. Depletion of ZMYND8 does not affect NuRD occupancy genome-wide and expression of NuRD/ZMYND8 target genes in steady-state asynchronous cells. However, ZMYND8 facilitates immediate recruitment of GATAD2A/NuRD to induced sites of DNA damage in a PAR-dependent manner. These results thus show that a specific substoichiometric interaction with a NuRD subunit paralogue provides unique functionality to a distinct NuRD subcomplex.
HostingRepository	PRIDE
AnnounceDate	2017-06-20
AnnouncementXML	Submission_2017-06-20_05:44:15.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Cornelia Spruijt
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	No PTMs are included in the dataset
Instrument	LTQ FT; Q Exactive; LTQ Orbitrap Velos

Revision	Datetime	Status	ChangeLog Entry
0	2016-03-24 00:43:24	ID requested
1	2016-10-10 00:29:22	announced
2	2016-10-18 02:21:18	announced	Updated publication reference for PubMed record(s): 27732854.
⏵ 3	2017-06-20 05:44:16	announced	Updated project metadata.
4	2017-10-24 01:53:25	announced	Updated project metadata.
5	2024-10-22 04:03:43	announced	2024-10-22: Updated project metadata.

Spruijt CG, Luijsterburg MS, Menafra R, Lindeboom RG, Jansen PW, Edupuganti RR, Baltissen MP, Wiegant WW, Voelker-Albert MC, Matarese F, Mensinga A, Poser I, Vos HR, Stunnenberg HG, van Attikum H, Vermeulen M, ZMYND8 Co-localizes with NuRD on Target Genes and Regulates Poly(ADP-Ribose)-Dependent Recruitment of GATAD2A/NuRD to Sites of DNA Damage. Cell Rep, 17(3):783-798(2016) [pubmed]

submitter keyword: ZMYND8, NuRD, epigenetics, GFP-affinity purification, SILAC, LFQ

Michiel Vermeulen
contact affiliation	Molecular Biology, RIMLS, Nijmegen, The Netherlands
contact email	michiel.vermeulen@science.ru.nl
lab head
Cornelia Spruijt
contact affiliation	RIMLS, RU, Nijmegen, The Netherlands
contact email	nellekespruijt@hotmail.com
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2016/10/PXD003856

PRIDE project URI

• PRIDE
  1. PXD003856
     1. Label: PRIDE project
     2. Name: ¬¬ZMYND8 co-localizes with NuRD on target genes and regulates poly-ADP-ribose dependent recruitment of GATAD2A/NuRD to sites of DNA damage