PXD003850-2

PXD003850 is an original dataset announced via ProteomeXchange.

Title	Glucose-regulated and drug perturbed beta-cell phosphoproteome reveals molecular mechanisms controlling insulin secretion
Description	Insulin-secreting beta cells play an essential role in maintaining physiological blood glucose levels, and their dysfunction leads to the development of diabetes. To elucidate the signaling events regulating insulin secretion, we applied a recently developed proteomics and phosphoproteomics workflow. We quantified the time-resolved phosphoproteome of beta cells following their exposure to glucose and in combination with small molecule compounds that promote insulin secretion. The quantitative phosphoproteome of 30,000 sites clustered into three main groups in concordance with the modulation of three key signaling pathways. In addition to their differential modulation of key cellular kinases, all compounds affected pathways regulating the cell cycle. A high-resolution time course revealed key novel regulatory sites in early and late insulin secretion, and unexpected connections to epigenetic regulation of gene expression. Our comprehensive and multi-parametric phosphoproteomics study reveals that control of insulin secretion is embedded in an unexpectedly broad and complex range of cellular functions.
HostingRepository	PRIDE
AnnounceDate	2017-10-24
AnnouncementXML	Submission_2017-10-24_02:16:01.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Francesca Sacco
SpeciesList	scientific name: Mus musculus (Mouse); NCBI TaxID: 10090; scientific name: Rattus norvegicus (Rat); NCBI TaxID: 10116;
ModificationList	phosphorylated residue
Instrument	Q Exactive

Revision	Datetime	Status	ChangeLog Entry
0	2016-03-23 08:01:39	ID requested
1	2016-12-13 00:50:56	announced
⏵ 2	2017-10-24 02:16:02	announced	Updated project metadata.

Sacco F, Humphrey SJ, Cox J, Mischnik M, Schulte A, Klabunde T, Sch, ä, fer M, Mann M, Glucose-regulated and drug-perturbed phosphoproteome reveals molecular mechanisms controlling insulin secretion. Nat Commun, 7():13250(2016) [pubmed]

curator keyword: Biomedical

submitter keyword: Beta cells, insulin secretion, phoshoproteome, islets, systems biology

Matthias Mann
contact affiliation	Max Plank Institute for Biochemistry
contact email	mmann@biochem.mpg.de
lab head
Francesca Sacco
contact affiliation	Max Plank Institute
contact email	fsacco@biochem.mpg.de
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2016/12/PXD003850

PRIDE project URI

• PRIDE
  1. PXD003850
     1. Label: PRIDE project
     2. Name: Glucose-regulated and drug perturbed beta-cell phosphoproteome reveals molecular mechanisms controlling insulin secretion