PXD003830 is an
original dataset announced via ProteomeXchange.
Dataset Summary
Title | Comparative genomic, proteomic and exoproteomic analyses of three Pseudomonas spp. Part 5 |
Description | Bacteria that inhabit the rhizosphere of agricultural crops can have a beneficial effect on crop growth, including through the solubilisation and remineralisation of complex forms of phosphorus (P). However[1], our understanding of the bacterial proteomic response to P stress is limited. Here, exoproteomic analysis of three Pseudomonas species was performed in unison[2] with proteomic analysis of Pseudomonas putida BIRD-1 (BIRD-1) grown [3]under P replete and P deplete conditions. Comparative exoproteomics revealed marked heterogeneity in the exoproteomes of each Pseudomonas species in response to low concentrations of P. In addition to well-characterised members of the PHO regulon such as alkaline phosphatases, several previously undiscovered proteins are responsive to phosphate depletion including putative nucleases, phosphotriesterases, putative phosphonate transporters and outer membrane proteins. Moreover, in BIRD-1, mutagenesis of the master regulator, phoBR, led us to confirm the addition of several novel PHO-dependent proteins. Our data expands knowledge of the Pseudomonas PHO regulon, including species that are frequently used as bioinoculants, opening up the potential for more efficient and complete use of soil complexed P. |
HostingRepository | PRIDE |
AnnounceDate | 2016-08-22 |
AnnouncementXML | Submission_2017-02-06_01:56:02.xml |
DigitalObjectIdentifier | https://dx.doi.org/10.6019/PXD003830 |
ReviewLevel | Peer-reviewed dataset |
DatasetOrigin | Original dataset |
RepositorySupport | Supported dataset by repository |
PrimarySubmitter | Alex Jones |
SpeciesList | scientific name: Pseudomonas putida (strain BIRD-1); NCBI TaxID: 931281; |
ModificationList | Oxidation; Carbamidomethyl |
Instrument | Orbitrap Fusion |
Dataset History
Revision | Datetime | Status | ChangeLog Entry |
0 | 2016-03-21 06:37:32 | ID requested | |
1 | 2016-08-22 10:26:01 | announced | |
⏵ 2 | 2017-02-06 01:56:04 | announced | Updated publication reference for PubMed record(s): 27233093. |
Publication List
Lidbury ID, Murphy AR, Scanlan DJ, Bending GD, Jones AM, Moore JD, Goodall A, Hammond JP, Wellington EM, Comparative genomic, proteomic and exoproteomic analyses of three Pseudomonas strains reveals novel insights into the phosphorus scavenging capabilities of soil bacteria. Environ Microbiol, 18(10):3535-3549(2016) [pubmed] |
Keyword List
curator keyword: Biological |
submitter keyword: exoproteome Pseudomonas putida BIRD pho mutant |
Contact List
Alex Jones |
contact affiliation | University of Warwick |
contact email | alex.jones@warwick.ac.uk |
lab head | |
Alex Jones |
contact affiliation | University of Warwick |
contact email | alex.jones@warwick.ac.uk |
dataset submitter | |
Full Dataset Link List
Dataset FTP location
NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2016/08/PXD003830 |
PRIDE project URI |
Repository Record List
[ + ]
[ - ]
- PRIDE
- PXD003830
- Label: PRIDE project
- Name: Comparative genomic, proteomic and exoproteomic analyses of three Pseudomonas spp. Part 5