PXD003800-1

PXD003800 is an original dataset announced via ProteomeXchange.

Title	Epstein-Barr virus protein EB2 stimulates translation initiation of mRNAs through direct interactions with both PABP and eIF4G
Description	Epstein-Barr virus (EBV) expresses several mRNAs produced from intronless genes that could be unfavorably translated in front of cellular spliced mRNAs. To overpass these limitations, the virus encodes a RNA-binding protein (RBP), EB2, which is already known to facilitate nuclear mRNAs export and to increase their translational yield. Here we show that EB2 binds both nuclear and cytoplasmic cap-binding complexes (respectively, CBC and eIF4F), and the poly(A)-binding protein (PABP) to enhance translation initiation of a given mRNP. Interestingly, such effect can be obtained only if EB2 was initially bound to the native mRNPs in the nucleus. We also demonstrate that the EB2-eIF4F-PABP association renders the translation of these mRNPs less sensitive to inhibitors of initiation. Taken together, our new data suggest that EB2 binds and stabilizes cap-binding complexes to increase mRNP translation and demonstrate the importance of the mRNP assembly process in the nucleus to promote translation in the cytoplasm.
HostingRepository	PRIDE
AnnounceDate	2024-10-08
AnnouncementXML	Submission_2024-10-08_11:34:33.748.xml
DigitalObjectIdentifier	https://dx.doi.org/10.6019/PXD003800
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Supported dataset by repository
PrimarySubmitter	Frederic DELOLME
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	No PTMs are included in the dataset
Instrument	LTQ XL ETD

Revision	Datetime	Status	ChangeLog Entry
0	2016-03-17 01:19:57	ID requested
⏵ 1	2024-10-08 11:34:34	announced

10.1128/JVI.01917-17;

10.6019/PXD003800;

curator keyword: Biomedical

submitter keyword: LC-MSMS, EB2-BMLF1,Epstein-Barr virus, translation

Frederic DELOLME
contact affiliation	UMS3444, Protein Science Facility, FRANCE
contact email	frederic.delolme@ibcp.fr
lab head
Frederic DELOLME
contact affiliation	CNRS UMS3444 Protein Science Facility
contact email	frederic.delolme@ibcp.fr
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD003800
     1. Label: PRIDE project
     2. Name: Epstein-Barr virus protein EB2 stimulates translation initiation of mRNAs through direct interactions with both PABP and eIF4G