PXD003784-1

PXD003784 is an original dataset announced via ProteomeXchange.

Title	PRICKLE1 .
Description	Identification of the protein associated to PRICKLE1. Components of the evolutionarily conserved developmental planar cell polarity (PCP) pathway were recently described to play a prominent role in cancer cell dissemination. However, the molecular mechanisms by which PCP molecules drive the spread of cancer cells remain largely unknown. PRICKLE1 encodes a PCP protein bound to the promigratory serine/threonine kinase MINK1. We identify RICTOR, a member of the mTORC2 complex, as a PRICKLE1-binding partner and show that the integrity of the PRICKLE1-MINK1-RICTOR complex is required for activation of AKT, regulation of focal adhesions and cancer cell migration. Disruption of the PRICKLE1-RICTOR interaction results in a strong impairment of breast cancer cell dissemination in xenograft assays. Finally, we show that up-regulation of PRICKLE1 in basal breast cancers, a subtype characterized by high metastatic potential, is associated with poor metastasis-free survival.
HostingRepository	PRIDE
AnnounceDate	2024-10-07
AnnouncementXML	Submission_2024-10-07_10:03:42.061.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Avais Daulat
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	monohydroxylated residue; iodoacetamide derivatized residue
Instrument	LTQ Orbitrap Velos

Revision	Datetime	Status	ChangeLog Entry
0	2016-03-14 04:43:59	ID requested
⏵ 1	2024-10-07 10:03:42	announced

10.1038/s41416-019-0448-z;

Daulat AM, Finetti P, Revinski D, Silveira Wagner M, Camoin L, Audebert S, Birnbaum D, Kodjabachian L, Borg JP, Bertucci F, ECT2 associated to PRICKLE1 are poor-prognosis markers in triple-negative breast cancer. Br J Cancer, 120(9):931-940(2019) [pubmed]

curator keyword: Biomedical

submitter keyword: RICTOR, MINK1,PRICKLE1

Avais M Daulat
contact affiliation	CRCM, Cell Polarity, Cell signalling and Cancer “Equipe labellisée Ligue Contre le Cancer”, Inserm,U1068, Marseille, F-13009, France Institut Paoli-Calmettes, Marseille, F-13009, France Aix-Marseille Université, F-13284, Marseille, France CNRS, UMR7258
contact email	avais.daulat@inserm.fr
lab head
Avais Daulat
contact affiliation	CRCM
contact email	avais.daulat@inserm.fr
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2022/03/PXD003784

PRIDE project URI

• PRIDE
  1. PXD003784
     1. Label: PRIDE project
     2. Name: PRICKLE1