PXD003777-3

PXD003777 is an original dataset announced via ProteomeXchange.

Title	Human Platelet PIP3 Signalosome
Description	Platelets are small, anucleate cells that play an essential role in haemostasis, but can contribute critically to the pathogenesis of cardiovascular disease. Platelets express all four Class I Phosphoinositide 3-kinase (PI3K) isoforms, with previous work revealing important roles for these enzymes in regulating platelet priming, activation and stable thrombus formation. Despite this, detailed mechanistic understanding of how these lipid kinases orchestrate these roles in platelets is limited. Class I PI3Ks predominantly regulate cell function through their catalytic product, the signalling phospholipid phosphatidylinositol-3,4,5-trisphosphate (PIP3), which coordinates the localisation and/or activity of a diverse range of binding proteins. The complete repertoire of these Class I PI3K effectors in platelets remains unknown, hampering current understanding of Class I PI3K-mediated regulation of platelet function. We therefore conducted a global, unbiased screen for PIP3-binding proteins in human platelets using affinity capture coupled to high resolution proteomic mass spectrometry. Thus, we present the first in depth analysis of the PIP3 signalosome of human platelets, revealing an extensive PIP3 interactome, including many proteins previously uncharacterised in this cell type. This work provides important new insights into how Class I PI3K shapes human platelet function.
HostingRepository	PRIDE
AnnounceDate	2017-06-06
AnnouncementXML	Submission_2018-05-23_05:47:06.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Tom Durrant
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	phosphorylated residue; monohydroxylated residue; iodoacetamide derivatized residue
Instrument	Orbitrap Fusion

Revision	Datetime	Status	ChangeLog Entry
0	2016-03-14 03:39:51	ID requested
1	2017-06-06 02:58:53	announced
2	2017-08-09 01:26:07	announced	Updated publication reference for DOI(s): 10.1182/bloodadvances.2017005173.
⏵ 3	2018-05-23 05:47:07	announced	Updated publication reference for DOI(s): 10.1182/bloodadvances.2017005173.
4	2023-11-14 08:48:54	announced	2023-11-14: Updated project metadata.

Durrant TN, Hutchinson JL, Heesom KJ, Anderson KE, Stephens LR, Hawkins PT, Marshall AJ, Moore SF, Hers I, signalosome analysis identifies DAPP1 as a negative regulator of GPVI-driven platelet function. Blood Adv, 1(14):918-932(2017) [pubmed]

10.1182/bloodadvances.2017005173;

curator keyword: Biomedical

submitter keyword: Platelet, Platelets, PIP3, PI3K, LC-MSMS

Ingeborg Hers
contact affiliation	Department of Physiology, Pharmacology & Neuroscience, University of Bristol, Bristol United Kingdom
contact email	i.hers@bristol.ac.uk
lab head
Tom Durrant
contact affiliation	University of Bristol
contact email	tom.durrant@bristol.ac.uk
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2017/06/PXD003777

PRIDE project URI

• PRIDE
  1. PXD003777
     1. Label: PRIDE project
     2. Name: Human Platelet PIP3 Signalosome