PXD003712-3

PXD003712 is an original dataset announced via ProteomeXchange.

Title	ATM INDUCES MACROD2 NUCLEAR EXPORT UPON DNA DAMAGE
Description	ADP-ribosylation is a dynamic post-translation modification that regulates the early phase of various DNA repair pathways by recruiting repair factors to chromatin. ADP-ribosylation levels are defined by the activities of transferases and hydrolases. Little is known about regulation of these enzymes except for that of the transferase ARTD1/PARP1. Among the hydrolases, MacroD2 contains a unique C-terminal region, which may regulate the enzymatic activity carried out by the N-terminal macrodomain. Our study shows that MacroD2 is exported from the nucleus upon DNA damage, and that this nuclear export is induced by ATM activity. In the MacroD2 C-terminal unstructured region we find two SQ/TQ motifs that are necessary for this regulation, arguing for a direct interaction with ATM. This study also shows that MacroD2 nuclear export restricts MacroD2 recruitment to the DNA damage site on the temporal scale, suggesting that the regulation of location determines a regulation of enzymatic activity.
HostingRepository	PRIDE
AnnounceDate	2024-10-22
AnnouncementXML	Submission_2024-10-22_04:14:07.030.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Andreas Schmidt
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	phosphorylated residue; monohydroxylated residue; iodoacetamide derivatized residue
Instrument	LTQ Orbitrap

Revision	Datetime	Status	ChangeLog Entry
0	2016-02-29 03:28:06	ID requested
1	2016-10-12 08:09:16	announced
2	2017-01-18 05:15:12	announced	Updated publication reference for PubMed record(s): 28069995.
⏵ 3	2024-10-22 04:14:07	announced	2024-10-22: Updated project metadata.

10.1093/nar/gkw904;

Golia B, Moeller GK, Jankevicius G, Schmidt A, Hegele A, Prei, ß, er J, Tran ML, Imhof A, Timinszky G, ATM induces MacroD2 nuclear export upon DNA damage. Nucleic Acids Res, 45(1):244-254(2017) [pubmed]

curator keyword: Biological

submitter keyword: ATM phosphorylation,DNA damage, nuclear export, regulation of ADP-ribosylation

Axel Imhof
contact affiliation	Zentrallabor für Proteinanalytik (Protein Analysis Unit), Biomedical Center, Ludwig-Maximilians-Universität München, Grosshadernerstr. 9, 82152 Planegg-Martinsried, Germany
contact email	imhof@lmu.de
lab head
Andreas Schmidt
contact affiliation	CECAD, University of Cologne, AG Krüger
contact email	A.Schmidt@med.uni-muenchen.de
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2016/10/PXD003712

PRIDE project URI

• PRIDE
  1. PXD003712
     1. Label: PRIDE project
     2. Name: ATM INDUCES MACROD2 NUCLEAR EXPORT UPON DNA DAMAGE