PXD003708-1

PXD003708 is an original dataset announced via ProteomeXchange.

Title	Phosphoproteome of a patient-matched cell line model of colon cancer metastasis
Description	The contributions of phosphorylation-mediated signaling networks to colon cancer metastasis are poorly defined. To better understand the role of constitutive signaling alterations in cancer progression, the global phosphoproteomes of the SW480 and SW620 cell lines were compared by LC-MS/MS. These patient-matched cell lines were derived from a primary colon tumor and a lymph node metastasis, respectively. Network analysis of the significantly altered phosphosites revealed differential regulation in cellular adhesion, mitosis, and mRNA translational machinery. Among the latter group were sites on phosphoproteins with known roles in mRNA biogenesis and splicing, transport through the nuclear pores, initiating translation, as well as mRNA stability and degradation. Though alterations in these processes have been associated with oncogenic transformation, control of mRNA stability has typically not been associated with cancer progression. Notably, the single phosphosite with the greatest relative change in SW620 was Ser2 on eIF2S2, suggesting that SW620 cells translate faster or with greater efficiency than SW480 cells. These broad changes in the regulation of translation also occur without over-expression of eIF4E. Altogether, this work shows that metastatic cells exhibit constitutive changes to the phosphoproteome, and that mRNA stability and translational efficiency may be important targets of deregulation during cancer progression.
HostingRepository	PRIDE
AnnounceDate	2017-01-06
AnnouncementXML	Submission_2017-01-06_08:02:00.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Alissa Schunter
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	phosphorylated residue
Instrument	Q Exactive

Revision	Datetime	Status	ChangeLog Entry
0	2016-02-29 01:28:56	ID requested
⏵ 1	2017-01-06 08:02:01	announced
2	2017-10-24 05:41:15	announced	Updated project metadata.
3	2024-10-22 04:14:10	announced	2024-10-22: Updated project metadata.

Schunter AJ, Yue X, Hummon AB, Phosphoproteomics of colon cancer metastasis: comparative mass spectrometric analysis of the isogenic primary and metastatic cell lines SW480 and SW620. Anal Bioanal Chem, 409(7):1749-1763(2017) [pubmed]

curator keyword: Biomedical

submitter keyword: phosphorylation, consitutive signaling, SW480, SW620, lymph node metastasis

Amanda B. Hummon
contact affiliation	University of Notre Dame, Indiana, USA Department of Chemistry and Biochemistry Harper Cancer Research Institute
contact email	ahummon@nd.edu
lab head
Alissa Schunter
contact affiliation	University of Notre Dame
contact email	aschunter@gmail.com
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD003708
     1. Label: PRIDE project
     2. Name: Phosphoproteome of a patient-matched cell line model of colon cancer metastasis