PXD003704-1

PXD003704 is an original dataset announced via ProteomeXchange.

Title	Thermal proteome profiling of MCF-7 breast cancer cell line
Description	Palbociclib (Ibrance, Pfizer) is a recent drug approved by the FDA for phase 3 clinical trials in treating estrogen-receptor-positive and HER2-negative breast cancer. In vitro, palbociclib, a selective inhibitor of CDK4 and CDK6, was shown to reduce cellular proliferation of breast cancer cell lines by blocking progression of cells from G1 into S phase of the cell cycle. While the primary targets of palbociclib have been deciphered, the molecular mechanisms leading to drug resistance as well as off-targets effects of palbociclib are not known. To identify new palbociclib targets we propose a quantitative mass spectrometry and a Cellular Thermal Shift Assay (CETSA) that works under the assumption that protein-drug interaction stabilises the protein thus, leading to an increase in thermostability
HostingRepository	PRIDE
AnnounceDate	2018-04-18
AnnouncementXML	Submission_2018-04-18_07:26:53.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	julien peltier
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	hydroxylated proline; monohydroxylated residue; deamidated residue; iodoacetamide derivatized residue
Instrument	Orbitrap Fusion ETD

Revision	Datetime	Status	ChangeLog Entry
0	2016-02-29 01:03:22	ID requested
⏵ 1	2018-04-18 07:26:54	announced
2	2023-11-14 08:48:53	announced	2023-11-14: Updated project metadata.

10.15252/embj.201798359;

curator keyword: Biomedical

submitter keyword: Palbocilcib, drug discovery, quantitative proteomics, TMT, LC-MSMS, Thermal proteome

Matthias Trost
contact affiliation	MRC Protein Phosphorylation Unit, University of Dundee, Dundee, United Kingdom
contact email	m.trost@dundee.ac.uk
lab head
julien peltier
contact affiliation	University of dundee
contact email	j.peltier@dundee.ac.uk
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2018/04/PXD003704

PRIDE project URI

• PRIDE
  1. PXD003704
     1. Label: PRIDE project
     2. Name: Thermal proteome profiling of MCF-7 breast cancer cell line