PXD003701-2

PXD003701 is an original dataset announced via ProteomeXchange.

Title	Urea artifacts interfere with immunopurification of lysine acetylation
Description	Urea-based buffers can induce carbamylation artefacts. Here we report that carbamylation of lysine residues resembles the structure of acetylated lysines (K-ac) and compete during K-ac immunoaffinity purification. We propose an alternative strategy using an ionic detergent which improves significantly the selectivity and efficiency of K-ac affinity immuno-purifications.
HostingRepository	PRIDE
AnnounceDate	2017-10-24
AnnouncementXML	Submission_2017-10-24_04:43:55.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Ana Martinez
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	carbamoylated residue; monohydroxylated residue; acetylated residue; iodoacetamide derivatized residue
Instrument	Bruker Daltonics maXis series

Revision	Datetime	Status	ChangeLog Entry
0	2016-02-26 04:13:00	ID requested
1	2017-01-11 02:36:56	announced
⏵ 2	2017-10-24 04:43:56	announced	Updated project metadata.

Martinez-Val A, Garcia F, Xim, é, nez-Emb, ú, n P, Mart, í, nez Teresa-Calleja A, Ibarz N, Ruppen I, Munoz J, Urea Artifacts Interfere with Immuno-Purification of Lysine Acetylation. J Proteome Res, 16(2):1061-1068(2017) [pubmed]

curator keyword: Biomedical

submitter keyword: Carbamyl, acetyl, urea, immunopurification, acetylation, carbamylation

Javier Muñoz Peralta
contact affiliation	Spanish National Cancer Research Centre (CNIO)
contact email	jmunozpe@cnio.es
lab head
Ana Martinez
contact affiliation	Spanish National Cancer Research Centre
contact email	amdelval@cnio.es
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2017/01/PXD003701

PRIDE project URI

• PRIDE
  1. PXD003701
     1. Label: PRIDE project
     2. Name: Urea artifacts interfere with immunopurification of lysine acetylation