PXD003676-1

PXD003676 is an original dataset announced via ProteomeXchange.

Title	Shotgun proteomics of a rice elongating internode at boosting stage
Description	Stem internodes of grasses function in mechanical support, transport, and, in some species are a major sink organ for carbon in the form of cell wall polymers. To establish the rice elongating internode as a model for secondary cell wall development, we conducted cell wall composition, proteomic and metabolomic analyses of the second rice internode at booting stage. We measured major secondary cell wall components along eight segments of an elongating internode. Cellulose, lignin, and xylose increase as a percentage of cell wall material from the younger to the older internode segments, indicating active cell wall synthesis. With the whole elongating internode, we measured peptides via liquid-chromatography mass spectrometry (LC-MS) following trypsin digestion of size fractionated proteins. This identified a total of 3249 protein groups with at least two unique peptides, including many glycosyltransferases, acyltransferases, glycohyrolases, cell wall-localized proteins, and protein kinases that have or may have functions in cell wall biosynthesis or remodeling. In addition, GO over-representation analysis and KO pathway analysis indicate many proteins involved in biosynthetic processes, especially the synthesis of secondary metabolites such as phenylpropanoids, flavonoids, and tepenoids. Therefore, we also used LC-MS to measure methanol-extracted secondary metabolites from the whole internode at the elongation stage and three post-elongation stages, and from leaf and root at the second post-elongation stage. The results indicate secondary metabolites in stems are distinct from those of roots and leaves, and show different profiles during stem development. This study fills a void of knowledge of proteomics and metabolomics data for grass stems, especially of rice, and provides baseline knowledge for more detailed studies of cell wall synthesis and other biological processes during internode development. This and future work is aimed at optimizing stem development and cell wall composition of grasses to improve agronomic properties and biofuel production.
HostingRepository	PRIDE
AnnounceDate	2017-08-04
AnnouncementXML	Submission_2017-08-04_04:56:17.xml
DigitalObjectIdentifier	https://dx.doi.org/10.6019/PXD003676
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Supported dataset by repository
PrimarySubmitter	Fan Lin
SpeciesList	scientific name: Oryza sativa (Rice); NCBI TaxID: 4530;
ModificationList	Phospho; Deamidated; Oxidation; Carbamidomethyl
Instrument	Synapt MS; LTQ Orbitrap

Revision	Datetime	Status	ChangeLog Entry
0	2016-02-23 01:07:27	ID requested
⏵ 1	2017-08-04 04:56:18	announced

Lin F, Williams BJ, Thangella PAV, Ladak A, Schepmoes AA, Olivos HJ, Zhao K, Callister SJ, Bartley LE, Proteomics Coupled with Metabolite and Cell Wall Profiling Reveal Metabolic Processes of a Developing Rice Stem Internode. Front Plant Sci, 8():1134(2017) [pubmed]

submitter keyword: rice, stem, internode, cell wall, HDMSE, LTQ Orbitrap XL

Laura E. Bartley
contact affiliation	Department of Microbiology and Plant Biology, University of Oklahoma. U.S.A.
contact email	lbartley@ou.edu
lab head
Fan Lin
contact affiliation	University of Oklahoma
contact email	fanlin@ou.edu
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD003676
     1. Label: PRIDE project
     2. Name: Shotgun proteomics of a rice elongating internode at boosting stage