PXD003670 is an
original dataset announced via ProteomeXchange.
Dataset Summary
Title | Carcinoma-associated fibroblasts stimulate cancer cell invasion by mechanically remodeling the basement membrane |
Description | The first step in metastasis is dissemination of tumor cells into the surrounding tissue, or stroma. In carcinomas, cancers of epithelial origin, tumor cells must first breach the basement membrane (BM), a network that encapsulates the tumor. As tumor progresses, other cell types that reside in the stroma, such as carcinoma-associated fibroblasts (CAFs) accumulate around the tumor. Whether CAFs can help cancer cells to breach the BM remains an open question. Here we show that CAFs promote cancer cell invasion by physically remodeling the BM. Using a novel 3D in vitro model we observed that primary CAFs isolated from colon cancer patients stimulate cancer cell invasion through a native mesenteric BM. This stimulated invasion is the result of the physical presence of CAFs rather than an increased invasive potential of cancer cells. In the presence of CAFs, cancer cells can invade the BM in a matrix metalloproteinase-independent manner. Using live imaging, we found that CAFs actively pull and stretch the BM, leading to the formation of gaps through which cancer cells can migrate. Notably, CAF-mediated gap-widening is independent of proteolysis but relies on actomyosin contractility. We propose that CAFs exert mechanical forces that alter the organization and the physical properties of the BM, making it permissive for cancer cell invasion. |
HostingRepository | PRIDE |
AnnounceDate | 2017-09-18 |
AnnouncementXML | Submission_2017-09-18_00:27:31.xml |
DigitalObjectIdentifier | |
ReviewLevel | Peer-reviewed dataset |
DatasetOrigin | Original dataset |
RepositorySupport | Unsupported dataset by repository |
PrimarySubmitter | Guillaume Arras |
SpeciesList | scientific name: Homo sapiens (Human); NCBI TaxID: 9606; |
ModificationList | monohydroxylated residue; acetylated residue; iodoacetamide derivatized residue |
Instrument | Orbitrap Fusion; LTQ Orbitrap |
Dataset History
Revision | Datetime | Status | ChangeLog Entry |
0 | 2016-02-22 00:46:09 | ID requested | |
⏵ 1 | 2017-09-18 00:27:32 | announced | |
2 | 2017-11-08 04:42:29 | announced | Updated publication reference for PubMed record(s): 28931556. |
3 | 2018-07-03 08:03:54 | announced | Updated publication reference for PubMed record(s): 28931556. |
Publication List
Dataset with its publication pending |
Keyword List
curator keyword: Biomedical |
submitter keyword: basement membrane, cell invasion, CAF, SILAC, secretome |
Contact List
Damarys Loew |
contact affiliation | Laboratoire de Spectrométrie de Masse Protéomique (LSMP), Institut Curie, PSL Research University, France |
contact email | damarys.loew@curie.fr |
lab head | |
Guillaume Arras |
contact affiliation | Institut Curie |
contact email | guillaume.arras@curie.fr |
dataset submitter | |
Full Dataset Link List
Dataset FTP location
NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2017/09/PXD003670 |
PRIDE project URI |
Repository Record List
[ + ]
[ - ]
- PRIDE
- PXD003670
- Label: PRIDE project
- Name: Carcinoma-associated fibroblasts stimulate cancer cell invasion by mechanically remodeling the basement membrane