PXD003657-1

PXD003657 is an original dataset announced via ProteomeXchange.

Title	Antibody-independent identification of bovine milk-derived peptides in breast-milk
Description	Exclusively breast-fed infants can exhibit clear signs of IgE or non IgE-mediated cow’s milk allergy. The definite characterization of dietary cow’s milk proteins (CMP) that survive the maternal digestive tract to be absorbed into the bloodstream and secreted into breast milk remains missing. The aim of this study was to assess the occurrence of CMP-derived peptides in breast milk, using antibody-independent methods. Using high performance liquid chromatography-high resolution mass spectrometry in blinded assays, we identified 11 cow’s milk-derived peptides, including two β-lactoglobulin (2 out 6 samples) and one αs1-casein (1 out 6 samples) fragments, in breast milk from mothers receiving a cup of bovine milk daily. The β-lactoglobulin (β-Lg) fragments, namely f42-54 and f42-57, were absent in milk from mothers who observed a strict dairy-free diet (6 samples). In contrast, neither intact nor hydrolyzed β-Lg was detected by Western blot or competitive ELISA tests. CMP-derived peptides rather than intact CMP may sensitize or elicit allergic responses in the neonate through mother’s milk. Immunologically active peptides from the maternal diet could be involved in priming the newborn’s immune system to drive tolerogenic response in neonates and infants.
HostingRepository	PRIDE
AnnounceDate	2016-07-19
AnnouncementXML	Submission_2016-07-19_03:48:11.xml
DigitalObjectIdentifier	https://dx.doi.org/10.6019/PXD003657
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Supported dataset by repository
PrimarySubmitter	Randall Robinson
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	dehydrated residue; phosphorylated residue; monohydroxylated residue; acetylated residue; deaminated residue
Instrument	Q Exactive

Revision	Datetime	Status	ChangeLog Entry
0	2016-02-17 03:01:17	ID requested
⏵ 1	2016-07-19 03:48:12	announced

Picariello G, Addeo F, Ferranti P, Nocerino R, Paparo L, Passariello A, Dallas DC, Robinson RC, Barile D, Canani RB, Antibody-independent identification of bovine milk-derived peptides in breast-milk. Food Funct, 7(8):3402-9(2016) [pubmed]

curator keyword: Biomedical

submitter keyword: human, bovine, milk, peptides, LC-MS/MS

Roberto Berni Canani
contact affiliation	Department of Translational Medical Science, University of Naples 'Federico II', Italy
contact email	berni@unina.it
lab head
Randall Robinson
contact affiliation	UC Davis
contact email	rcrobinson@ucdavis.edu
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD003657
     1. Label: PRIDE project
     2. Name: Antibody-independent identification of bovine milk-derived peptides in breast-milk