PXD003621-1

PXD003621 is an original dataset announced via ProteomeXchange.

Title	Prolyl hydroxylation regulates protein degradation, synthesis and splicing in human induced pluripotent stem cell-derived cardiomyocytes
Description	Protein hydroxylases are oxygen and alpha-ketoglutarate-dependent enzymes that catalyze hydroxylation of amino acids such as proline, thus linking oxygen and metabolism to enzymatic activity. Prolyl hydroxylation is a dynamic post-translational modification that regulates protein stability and protein-protein interactions; however, the extent of this modification is largely uncharacterized. The goals of this study are to investigate the biological consequences of prolyl hydroxylation and to identify new targets which undergo prolyl hydroxylation in human cardiomyocytes.
HostingRepository	PRIDE
AnnounceDate	2016-05-06
AnnouncementXML	Submission_2016-05-06_06:50:17.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Andrea Stoehr
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	6x(13)C labeled residue; monohydroxylated residue; deamidated residue; iodoacetamide derivatized residue
Instrument	Thermo Scientific instrument model

Revision	Datetime	Status	ChangeLog Entry
0	2016-02-12 02:18:02	ID requested
⏵ 1	2016-05-06 06:50:21	announced
2	2024-10-22 04:09:55	announced	2024-10-22: Updated project metadata.

Stoehr A, Yang Y, Patel S, Evangelista AM, Aponte A, Wang G, Liu P, Boylston J, Kloner PH, Lin Y, Gucek M, Zhu J, Murphy E, Prolyl hydroxylation regulates protein degradation, synthesis, and splicing in human induced pluripotent stem cell-derived cardiomyocytes. Cardiovasc Res, 110(3):346-58(2016) [pubmed]

curator keyword: Biomedical

submitter keyword: LC-MS/MS, iPSC-derived cardiomyocytes, prolyl hydroxylation, amino acid labeling

Elizabeth (Tish) Murphy
contact affiliation	NIH/NHLBI Cardiac Physiology Systems Biology Center Bethesda, MD, USA
contact email	murphy1@nhlbi.nih.gov
lab head
Andrea Stoehr
contact affiliation	NIH/NHLBI
contact email	andrea.stoehr@nih.gov
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2016/05/PXD003621

PRIDE project URI

• PRIDE
  1. PXD003621
     1. Label: PRIDE project
     2. Name: Prolyl hydroxylation regulates protein degradation, synthesis and splicing in human induced pluripotent stem cell-derived cardiomyocytes