PXD003599-3

PXD003599 is an original dataset announced via ProteomeXchange.

Title	HVint validation by AP-MS analysis of HSV-1 VP26
Description	Human herpesviruses are widespread human pathogens with a remarkable impact on worldwide public health. Despite intense decades of research, the molecular details in many aspects of their function remain to be fully characterized. To unravel the details of how these viruses operate a thorough understanding of the relationships between the viral protein components is key. Here, we have created HVint, a novel protein-protein intra-viral interaction database for herpes simplex virus type 1 (HSV-1), which integrates data from five external sources. The coverage of the initial interactome was enlarged using evolutionary information, which integrates computational predictions for potential novel interactions in HSV-1. As an experimental validation for these predicted interactions, affinity purification mass spectrometry (AP-MS) was performed for the HSV-1 capsid protein, VP26. The VP26 interaction data provided experimental evidence supporting the interactions with pUL31 and pUL40, which were computationally predicted as direct partners. Other indirect interactions within the predicted network, such as pUL32 and pUL21, were also present in the AP-MS experiment. For access to the complete HVint network, we have developed a user-friendly and interactive web interface. Our approach demonstrates the power of computational predictions to assist in the design of targeted experiments for the discovery of novel protein-protein interactions.
HostingRepository	PRIDE
AnnounceDate	2017-10-24
AnnouncementXML	Submission_2017-10-24_04:07:02.xml
DigitalObjectIdentifier	https://dx.doi.org/10.6019/PXD003599
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Supported dataset by repository
PrimarySubmitter	T Greco
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	Ammonia-loss; Phospho; Deamidated; Dehydrated; Oxidation; Acetyl; Carbamidomethyl
Instrument	LTQ Orbitrap Velos

Revision	Datetime	Status	ChangeLog Entry
0	2016-02-09 05:53:31	ID requested
1	2016-07-15 00:04:43	announced
2	2017-03-04 00:30:27	announced	Updated project metadata.
⏵ 3	2017-10-24 04:07:03	announced	Updated project metadata.
4	2024-10-22 04:27:46	announced	2024-10-22: Updated project metadata.

Ashford P, Hernandez A, Greco TM, Buch A, Sodeik B, Cristea IM, Gr, ü, newald K, Shepherd A, Topf M, HVint: A Strategy for Identifying Novel Protein-Protein Interactions in Herpes Simplex Virus Type 1. Mol Cell Proteomics, 15(9):2939-53(2016) [pubmed]

submitter keyword: protein interactions, herpes simplex virus, virology, interactome, human, Orbitrap Velos

Ileana Cristea
contact affiliation	Department of Molecular Biology, Princeton University
contact email	icristea@princeton.edu
lab head
T Greco
contact affiliation	Molecular Biology
contact email	tgreco@princeton.edu
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2017/02/PXD003599

PRIDE project URI

• PRIDE
  1. PXD003599
     1. Label: PRIDE project
     2. Name: HVint validation by AP-MS analysis of HSV-1 VP26