PXD003569 is an
original dataset announced via ProteomeXchange.
Dataset Summary
Title | Stoichiometry and absolute abundance of proteins in the Bacillus cereus spore insoluble fraction determined using a QconCAT approach |
Description | Spores of Bacillus cereus pose a threat to food safety due to their high resistance to the heat or acid treatments commonly used to make food microbially safe. As spores survive these treatments and later resume growth either on foodstuffs or, after ingestion, upon entering the gut they are capable of producing toxins which cause either vomiting or diarrhea respectively. The outer layers of the spore consist primarily of proteins which may serve as potential biomarkers for detection. In order to quantify proteins in the insoluble fraction of the spore coat, a proteomics approach using a QconCAT reference standard was employed which is the first time this is used in a heterogeneous system. This allowed us to determine the abundance of 21 proteins which spanned across three orders of magnitude and together covered 5.66% ±0.51 of the total spore weight. Classification by abundance resulted in functional characterisation of the protein BC_0987, renamed SasS. Furthermore, protein stoichiometry and determination of the abundance of, for instance, germination mediating enzymes provides useful information for model development. And finally, the four most abundant proteins, CotB1, CotB2, CotX and InhA, are presented to be biomarker candidates containing immunogenic epitopes as predicted by the SVMTriP algorithm and being conveniently located in the outermost layers of the spore. |
HostingRepository | PRIDE |
AnnounceDate | 2018-10-26 |
AnnouncementXML | Submission_2018-10-26_01:50:31.xml |
DigitalObjectIdentifier | |
ReviewLevel | Peer-reviewed dataset |
DatasetOrigin | Original dataset |
RepositorySupport | Unsupported dataset by repository |
PrimarySubmitter | Sacha Stelder |
SpeciesList | scientific name: Bacillus cereus; NCBI TaxID: 1396; |
ModificationList | No PTMs are included in the dataset |
Instrument | apex ultra |
Dataset History
Revision | Datetime | Status | ChangeLog Entry |
0 | 2016-02-03 06:20:58 | ID requested | |
⏵ 1 | 2018-10-26 01:50:32 | announced | |
Publication List
Stelder SK, Benito de Moya C, Hoefsloot HCJ, de Koning LJ, Brul S, de Koster CG, Stoichiometry, Absolute Abundance, and Localization of Proteins in the Bacillus cereus Spore Coat Insoluble Fraction Determined Using a QconCAT Approach. J Proteome Res, 17(2):903-917(2018) [pubmed] |
Keyword List
curator keyword: Biomedical |
submitter keyword: Bacillus cereus, spores, quantitative proteomics, QconCAT |
Contact List
Chris Gerdinus de Koster |
contact affiliation | Mass Spectrometry of Biomacromolecules, Swammerdam Institute for Life Sciences, University of Amsterdam, the Netherlands |
contact email | c.g.dekoster@uva.nl |
lab head | |
Sacha Stelder |
contact affiliation | University of Amsterdam |
contact email | s.k.stelder@uva.nl |
dataset submitter | |
Full Dataset Link List
Dataset FTP location
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PRIDE project URI |
Repository Record List
[ + ]
[ - ]
- PRIDE
- PXD003569
- Label: PRIDE project
- Name: Stoichiometry and absolute abundance of proteins in the Bacillus cereus spore insoluble fraction determined using a QconCAT approach