PXD003558-2

PXD003558 is an original dataset announced via ProteomeXchange.

Title	Global analysis of impact of Atg5 mutation on steady-state protein levels
Description	This experiment analyzes the global impact of Atg5-/- mutation on steady-state protein levels by a standard SILAC experiment. WT+vector cells were grown in isotopically labeled media for multiple passages in order to fully label the proteome. Unlabeled Atg5-/- cells and labeled WT+vector cells were grown to confluency and protein extracts from quiescent cultures were combined at a 1:1 ratio and analyzed by LC-MS/MS. In this experiment, ratios of labeled to unlabeled spectra report on changes in steady-state expression levels of proteins induced by impairment of autophagy. Table of files Cells Fractionation Raw Data Filenames Wildtype & Atg5-/- Yes SILAC_(1-8).raw (8 fractions)
HostingRepository	PRIDE
AnnounceDate	2024-10-22
AnnouncementXML	Submission_2024-10-22_04:28:17.165.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Tian Zhang
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	6x(13)C labeled residue; acetylated residue; monohydroxylated residue; iodoacetamide derivatized residue
Instrument	Q Exactive

Revision	Datetime	Status	ChangeLog Entry
0	2016-02-02 01:16:43	ID requested
1	2016-06-13 23:51:22	announced
⏵ 2	2024-10-22 04:28:25	announced	2024-10-22: Updated project metadata.

10.1016/j.celrep.2016.02.040;

Zhang T, Shen S, Qu J, Ghaemmaghami S, Global Analysis of Cellular Protein Flux Quantifies the Selectivity of Basal Autophagy. Cell Rep, 14(10):2426-39(2016) [pubmed]

curator keyword: Biological

submitter keyword: steady state protein level, Atg5,human primary fibroblasts

Sina Ghaemmaghami
contact affiliation	Department of Biology, University of Rochester, NY, USA
contact email	sghaemma@ur.rochester.edu
lab head
Tian Zhang
contact affiliation	UNIVERSITY OF ROCHESTER
contact email	tzhang24@ur.rochester.edu
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2016/06/PXD003558

PRIDE project URI

• PRIDE
  1. PXD003558
     1. Label: PRIDE project
     2. Name: Global analysis of impact of Atg5 mutation on steady-state protein levels