PXD003532-2

PXD003532 is an original dataset announced via ProteomeXchange.

Title	Proteomic Analysis of Loricrin Knockout Mouse Epidermis IBAQ
Description	The cross-linked envelope of the mammalian epidermal corneocyte is important as a scaffold for assembly of the lipid barrier of the epidermis. Illustrating its importance, deficient envelope cross-linking by keratinocyte transglutaminase (TGM1) is a major cause of the autosomal recessive congenital ichthyoses, characterized by barrier defects. Expectations that loss of some of the known envelope protein components would also confer an ichthyosis phenotype have been difficult to demonstrate. To help rationalize this observation with one major component, the protein profile of epidermis from loricrin knockout mice has been compared to that of the wild type. Despite the mild phenotype of the knockout, some 40 proteins have been seen to be incorporated into envelope material to significantly different extents. Nearly half of these proteins were also seen to be incorporated to similarly altered extents into the disulfide bonded keratin network of the corneocyte. The results suggest that loss of loricrin alters their incorporation into envelopes as a consequence of protein-protein interactions during cell maturation. Mass spectrometric protein profiling revealed that keratin 1, keratin 10 and filaggrin are prominent envelope components and that dozens of other proteins are minor components. This finding helps rationalize the potential formation of functional envelopes despite loss of a single component due to availability of many alternative transglutaminase substrates.
HostingRepository	PRIDE
AnnounceDate	2017-10-24
AnnouncementXML	Submission_2017-10-24_03:04:23.xml
DigitalObjectIdentifier	https://dx.doi.org/10.6019/PXD003532
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Supported dataset by repository
PrimarySubmitter	Brett Phinney
SpeciesList	scientific name: Mus musculus (Mouse); NCBI TaxID: 10090;
ModificationList	Deamidated; Oxidation; Acetyl; Carbamidomethyl
Instrument	Q Exactive

Revision	Datetime	Status	ChangeLog Entry
0	2016-01-27 02:31:25	ID requested
1	2016-12-21 01:47:46	announced
⏵ 2	2017-10-24 03:04:24	announced	Updated project metadata.

Rice RH, Durbin-Johnson BP, Ishitsuka Y, Salemi M, Phinney BS, Rocke DM, Roop DR, Proteomic Analysis of Loricrin Knockout Mouse Epidermis. J Proteome Res, 15(8):2560-6(2016) [pubmed]

curator keyword: Biological

submitter keyword: Loricrin, Mouse, Epidermis

Brett Phinney
contact affiliation	UC Davis
contact email	bsphinney@ucdavis.edu
lab head
Brett Phinney
contact affiliation	UC Davis Proteomics Core
contact email	bsphinney@ucdavis.edu
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2016/12/PXD003532

PRIDE project URI

• PRIDE
  1. PXD003532
     1. Label: PRIDE project
     2. Name: Proteomic Analysis of Loricrin Knockout Mouse Epidermis IBAQ