PXD003519 is an
original dataset announced via ProteomeXchange.
Dataset Summary
| Title | Single Cell Immuno-laser Microdissection Coupled to Label-free Proteomics to Reveal the Proteotypes of Human Brain Cells After Ischemia |
| Description | Cerebral ischemia entails rapid tissue damage in the affected brain area causing devastating neurological disorders. The use of biomarkers in stroke might have a major impact in patients’ management, both in the diagnosis and the prognosis. Thus, the analysis of the brain proteome is a straightforward approach for the identification of specific biomarkers and for the detection of potential new therapeutic targets for neuroprotection or recovery strategies. In this study, post-mortem brain slices from ischemic stroke patients were obtained corresponding to infarcted (IC) and contralateral (CL) areas. By means of laser microdissection, neurons and blood brain barrier structures (BBB) were isolated and analyzed in a label-free manner on a LTQ Orbitrap Velos Pro MS following a gas phase fractionation approach for precursor ion selection. Ninety one proteins were identified only in neurons, 261 proteins only in BBB and 260 proteins in both cell types (peptides≥2). Gene ontology analyses revealed the alteration of metabolic processes after brain ischemia and also changes regarding catalytic activity and binding. Antioxidant activity and enzyme regulator activity appeared as cell-specific altered molecular functions. A total of 25 proteins showing p<0.05 and fold-change>|2| between IC and CL areas were considered significant in this study: 11 in neurons, 11 in BBB and 3 in both cell types. Twelve of these proteins were selected as candidates and analyzed by immunohistochemistry in independent brains. The MS findings were verified for neuronal SAHH2 and SRSF1 and for EAA2 in both cell types. In addition, SAHH2 showed its potential as prognostic biomarker when analyzed early in plasma of ischemic stroke patients. Therefore, our results will contribute to increase the knowledge about the molecular mechanisms of ischemic stroke pathology and launch new proteins that might represent putative biomarkers of brain ischemia or therapeutic targets. |
| HostingRepository | PRIDE |
| AnnounceDate | 2024-10-22 |
| AnnouncementXML | Submission_2024-10-22_04:40:03.988.xml |
| DigitalObjectIdentifier | https://dx.doi.org/10.6019/PXD003519 |
| ReviewLevel | Peer-reviewed dataset |
| DatasetOrigin | Original dataset |
| RepositorySupport | Supported dataset by repository |
| PrimarySubmitter | Víctor Llombart |
| SpeciesList | scientific name: Homo sapiens (Human); NCBI TaxID: 9606; |
| ModificationList | Oxidation; Carbamidomethyl |
| Instrument | LTQ Orbitrap |
Dataset History
| Revision | Datetime | Status | ChangeLog Entry |
|---|
| 0 | 2016-01-25 03:49:16 | ID requested | |
| 1 | 2017-12-04 02:09:59 | announced | |
| ⏵ 2 | 2024-10-22 04:40:04 | announced | 2024-10-22: Updated project metadata. |
Publication List
| 10.6019/PXD003519; |
| 10.1074/mcp.ra117.000419; |
| Garc, í, a-Berrocoso T, Llombart V, Col, à, s-Camp, à, s L, Hainard A, Licker V, Penalba A, Ramiro L, Simats A, Bustamante A, Mart, í, nez-Saez E, Canals F, Sanchez JC, Montaner J, Single Cell Immuno-Laser Microdissection Coupled to Label-Free Proteomics to Reveal the Proteotypes of Human Brain Cells After Ischemia. Mol Cell Proteomics, 17(1):175-189(2018) [pubmed] |
Keyword List
| curator keyword: Biomedical |
| submitter keyword: proteomics, label-free,stroke, brain ischemia, laser micro-dissection, biomarker |
Contact List
| Joan Montaner |
|---|
| contact affiliation | Neurovascular Research Laboratory, Vall Hebron Institute of Research, Barcelona, Spain. |
| contact email | montaner.villalonga@gmail.com |
| lab head | |
| Víctor Llombart |
|---|
| contact affiliation | Vall Hebrón Institute of Research |
| contact email | llombs@hotmail.com |
| dataset submitter | |
Full Dataset Link List
Dataset FTP location
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| PRIDE project URI |
Repository Record List
[ + ]
[ - ]
- PRIDE
- PXD003519
- Label: PRIDE project
- Name: Single Cell Immuno-laser Microdissection Coupled to Label-free Proteomics to Reveal the Proteotypes of Human Brain Cells After Ischemia
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