PXD003509 is an
original dataset announced via ProteomeXchange.
Dataset Summary
Title | Complexome profiling of membrane fraction from Nox4 overexpressing HEK293 cells |
Description | Within the family of NADPH oxidases, Nox4 is unique as it is predominantly localized in the endoplasmic reticulum, has constitutive activity and generates H2O2. We hypothesize that these features are consequences of a so far unidentified Nox4-interacting protein. Blue native gel electrophoresis of solubilized membranes from HEK293 cells (overexpressing Nox4) separated macromolecular complexes containing Nox4. The combination of native gel electrophoresis and quantitative mass spectrometry was applied to identify proteins that co-migrate with Nox4. In addition, the data set contains information about macromolecular protein complexes in human cellular membranes that are stable to the solubilization with digitonin. |
HostingRepository | PRIDE |
AnnounceDate | 2017-04-07 |
AnnouncementXML | Submission_2017-04-06_23:29:51.xml |
DigitalObjectIdentifier | |
ReviewLevel | Peer-reviewed dataset |
DatasetOrigin | Original dataset |
RepositorySupport | Unsupported dataset by repository |
PrimarySubmitter | Ilka Wittig |
SpeciesList | scientific name: Homo sapiens (Human); NCBI TaxID: 9606; |
ModificationList | iodoacetamide derivatized residue |
Instrument | LTQ Orbitrap |
Dataset History
Revision | Datetime | Status | ChangeLog Entry |
0 | 2016-01-22 01:14:04 | ID requested | |
⏵ 1 | 2017-04-06 23:29:53 | announced | |
Publication List
Prior KK, Wittig I, Leisegang MS, Groenendyk J, Weissmann N, Michalak M, Jansen-D, ü, rr P, Shah AM, Brandes RP, The Endoplasmic Reticulum Chaperone Calnexin Is a NADPH Oxidase NOX4 Interacting Protein. J Biol Chem, 291(13):7045-59(2016) [pubmed] |
Keyword List
curator keyword: Biological |
submitter keyword: Complexome profiling, LC-MSMS, macromolecular complexes, Blue native electrophoresis |
Contact List
Ralf Brandes |
contact affiliation | Institut für Kardiovaskuläre Physiologie, Goethe-Universität, Frankfurt am Main, Germany |
contact email | brandes@vrc.uni-frankfurt.de |
lab head | |
Ilka Wittig |
contact affiliation | Functional Proteomics, Goethe University, Frankfurt am Main , Germany |
contact email | wittig@med.uni-frankfurt.de |
dataset submitter | |
Full Dataset Link List
Dataset FTP location
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PRIDE project URI |
Repository Record List
[ + ]
[ - ]
- PRIDE
- PXD003509
- Label: PRIDE project
- Name: Complexome profiling of membrane fraction from Nox4 overexpressing HEK293 cells