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PXD003509-1

PXD003509 is an original dataset announced via ProteomeXchange.

Dataset Summary
TitleComplexome profiling of membrane fraction from Nox4 overexpressing HEK293 cells
DescriptionWithin the family of NADPH oxidases, Nox4 is unique as it is predominantly localized in the endoplasmic reticulum, has constitutive activity and generates H2O2. We hypothesize that these features are consequences of a so far unidentified Nox4-interacting protein. Blue native gel electrophoresis of solubilized membranes from HEK293 cells (overexpressing Nox4) separated macromolecular complexes containing Nox4. The combination of native gel electrophoresis and quantitative mass spectrometry was applied to identify proteins that co-migrate with Nox4. In addition, the data set contains information about macromolecular protein complexes in human cellular membranes that are stable to the solubilization with digitonin.
HostingRepositoryPRIDE
AnnounceDate2017-04-07
AnnouncementXMLSubmission_2017-04-06_23:29:51.xml
DigitalObjectIdentifier
ReviewLevelPeer-reviewed dataset
DatasetOriginOriginal dataset
RepositorySupportUnsupported dataset by repository
PrimarySubmitterIlka Wittig
SpeciesList scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationListiodoacetamide derivatized residue
InstrumentLTQ Orbitrap
Dataset History
RevisionDatetimeStatusChangeLog Entry
02016-01-22 01:14:04ID requested
12017-04-06 23:29:53announced
Publication List
Prior KK, Wittig I, Leisegang MS, Groenendyk J, Weissmann N, Michalak M, Jansen-D, ü, rr P, Shah AM, Brandes RP, The Endoplasmic Reticulum Chaperone Calnexin Is a NADPH Oxidase NOX4 Interacting Protein. J Biol Chem, 291(13):7045-59(2016) [pubmed]
Keyword List
curator keyword: Biological
submitter keyword: Complexome profiling, LC-MSMS, macromolecular complexes, Blue native electrophoresis
Contact List
Ralf Brandes
contact affiliationInstitut für Kardiovaskuläre Physiologie, Goethe-Universität, Frankfurt am Main, Germany
contact emailbrandes@vrc.uni-frankfurt.de
lab head
Ilka Wittig
contact affiliationFunctional Proteomics, Goethe University, Frankfurt am Main , Germany
contact emailwittig@med.uni-frankfurt.de
dataset submitter
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Dataset FTP location
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PRIDE project URI
Repository Record List
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