PXD003509-1

PXD003509 is an original dataset announced via ProteomeXchange.

Title	Complexome profiling of membrane fraction from Nox4 overexpressing HEK293 cells
Description	Within the family of NADPH oxidases, Nox4 is unique as it is predominantly localized in the endoplasmic reticulum, has constitutive activity and generates H2O2. We hypothesize that these features are consequences of a so far unidentified Nox4-interacting protein. Blue native gel electrophoresis of solubilized membranes from HEK293 cells (overexpressing Nox4) separated macromolecular complexes containing Nox4. The combination of native gel electrophoresis and quantitative mass spectrometry was applied to identify proteins that co-migrate with Nox4. In addition, the data set contains information about macromolecular protein complexes in human cellular membranes that are stable to the solubilization with digitonin.
HostingRepository	PRIDE
AnnounceDate	2017-04-07
AnnouncementXML	Submission_2017-04-06_23:29:51.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Ilka Wittig
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	iodoacetamide derivatized residue
Instrument	LTQ Orbitrap

Revision	Datetime	Status	ChangeLog Entry
0	2016-01-22 01:14:04	ID requested
⏵ 1	2017-04-06 23:29:53	announced

Prior KK, Wittig I, Leisegang MS, Groenendyk J, Weissmann N, Michalak M, Jansen-D, ü, rr P, Shah AM, Brandes RP, The Endoplasmic Reticulum Chaperone Calnexin Is a NADPH Oxidase NOX4 Interacting Protein. J Biol Chem, 291(13):7045-59(2016) [pubmed]

curator keyword: Biological

submitter keyword: Complexome profiling, LC-MSMS, macromolecular complexes, Blue native electrophoresis

Ralf Brandes
contact affiliation	Institut für Kardiovaskuläre Physiologie, Goethe-Universität, Frankfurt am Main, Germany
contact email	brandes@vrc.uni-frankfurt.de
lab head
Ilka Wittig
contact affiliation	Functional Proteomics, Goethe University, Frankfurt am Main , Germany
contact email	wittig@med.uni-frankfurt.de
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2017/04/PXD003509

PRIDE project URI

• PRIDE
  1. PXD003509
     1. Label: PRIDE project
     2. Name: Complexome profiling of membrane fraction from Nox4 overexpressing HEK293 cells