PXD003489-2

PXD003489 is an original dataset announced via ProteomeXchange.

Title	SILAC-based proteomic profiling of the human colon cancer cell line LS 180 in response to exogenous galectin-4
Description	Galectins constitute a family of ß-galactoside binding proteins that translate sugar-encoded signals of cell surface glycoconjugates into biological effects. The expression pattern of different human galectins changes during tissue development and is altered at sites of inflammation and tumor. Galectins have been known to be involved in colorectal cancer development, progression and metastasis. Galectin-4 has already been reported to function as tumor suppressor in this type of malignancy. However, its detailed impact on the tumor phenotype is still unknown. Our previous work indicates that galectin-4 inhibits cell proliferation and induces morphological changes in analyzed human colon cancer cell lines, including LS 180.
HostingRepository	PRIDE
AnnounceDate	2017-10-24
AnnouncementXML	Submission_2017-10-24_03:26:03.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Malwina Michalak
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	monohydroxylated residue; deamidated residue; iodoacetamide derivatized residue
Instrument	LTQ Orbitrap

Revision	Datetime	Status	ChangeLog Entry
0	2016-01-20 01:33:26	ID requested
1	2017-01-03 03:35:29	announced
⏵ 2	2017-10-24 03:26:05	announced	Updated project metadata.
3	2024-10-22 04:28:42	announced	2024-10-22: Updated project metadata.

Michalak M, Warnken U, Andr, é S, Schn, ö, lzer M, Gabius HJ, Kopitz J, Detection of Proteome Changes in Human Colon Cancer Induced by Cell Surface Binding of Growth-Inhibitory Human Galectin-4 Using Quantitative SILAC-Based Proteomics. J Proteome Res, 15(12):4412-4422(2016) [pubmed]

curator keyword: Biomedical

submitter keyword: Human, Colon cancer, LS 180, Galectin-4, SILAC, LC-MSMS

Jürgen Kopitz
contact affiliation	Department of Applied Tumor Biology, Institute of Pathology, University Hospital Heidelberg, Germany
contact email	juergen.kopitz@med.uni-heidelberg.de
lab head
Malwina Michalak
contact affiliation	Institute of Pathology, University Hospital Heidelberg
contact email	malwina.michalak@med.uni-heidelberg.de
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2016/12/PXD003489

PRIDE project URI

• PRIDE
  1. PXD003489
     1. Label: PRIDE project
     2. Name: SILAC-based proteomic profiling of the human colon cancer cell line LS 180 in response to exogenous galectin-4