PXD003468-2

PXD003468 is an original dataset announced via ProteomeXchange.

Title	Incorporation of nonproteinogenic amino acids into bacterial proteome analyzed by unbiased quantitative MS of protein modifications
Description	To study incorporation of nonproteinogenic amino acids in bacterial proteome we performed a global, unbiased protein modification analysis of the E. coli K12 strain with defective editing mechanism of the leucyl tRNA synthetase (LeuRS), which in addition to leucine incorporates a nonproteinogenic amino acid norvaline. We measured widespread loss of methyl groups at leucine positions in the LeuRS mutant, indicative of norvaline incorporation. We performed a Super-SILAC experiment and established that under microaerobic conditions norvaline misincorporation reaches a maximum approximately 10 hours after entry into stationary phase, when up to 10% of all measured leucines are substituted with norvaline.
HostingRepository	PRIDE
AnnounceDate	2024-10-22
AnnouncementXML	Submission_2024-10-22_04:29:25.417.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Maja Semanjski
SpeciesList	scientific name: Escherichia coli; NCBI TaxID: 562;
ModificationList	No PTMs are included in the dataset
Instrument	Q Exactive; LTQ Orbitrap Elite

Revision	Datetime	Status	ChangeLog Entry
0	2016-01-15 05:40:41	ID requested
1	2016-07-11 06:08:00	announced
⏵ 2	2024-10-22 04:29:33	announced	2024-10-22: Updated project metadata.

10.1038/srep28631;

Cvetesic N, Semanjski M, Soufi B, Krug K, Gruic-Sovulj I, Macek B, Proteome-wide measurement of non-canonical bacterial mistranslation by quantitative mass spectrometry of protein modifications. Sci Rep, 6():28631(2016) [pubmed]

curator keyword: Biological

submitter keyword: Mistranslation, tRNA synthetase, norvaline, leucine, nonproteinogenic amino acids, Orbitrap

Boris Macek
contact affiliation	Proteome Center Tuebingen, Interfaculty Institute for Cell Biology, University of Tuebingen, Tuebingen, Germany
contact email	boris.macek@uni-tuebingen.de
lab head
Maja Semanjski
contact affiliation	Proteome Center Tuebingen, Tuebingen, Germany
contact email	maja.semanjski@ifiz.uni-tuebingen.de
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2016/07/PXD003468

PRIDE project URI

• PRIDE
  1. PXD003468
     1. Label: PRIDE project
     2. Name: Incorporation of nonproteinogenic amino acids into bacterial proteome analyzed by unbiased quantitative MS of protein modifications