PXD003462-2

PXD003462 is an original dataset announced via ProteomeXchange.

Title	Quantitative analysis of the supernatant from native and transfected CHO cells using iTRAQ 8-plex technique
Description	We employed UPLC-MS/MS with iTRAQ 8-plex labeling to quantitatively analyze the supernatant produced by two Chinese hamster ovary (CHO) cell lines (CHO K1SV and CHO CAT-S). In each case, the supernatant from the host and three transfected clones were analyzed at days 5, 7, and 10 of culture. A total of eight iTRAQ 8-plex experiments were performed.
HostingRepository	PRIDE
AnnounceDate	2024-10-22
AnnouncementXML	Submission_2024-10-22_04:27:26.335.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Guijie Zhu
SpeciesList	scientific name: Cricetulus griseus (Chinese hamster) (Cricetulus barabensis griseus); NCBI TaxID: 10029;
ModificationList	iTRAQ8plex-116 reporter+balance reagent acylated residue; monohydroxylated residue; iodoacetamide derivatized residue; deamidated residue
Instrument	Q Exactive

Revision	Datetime	Status	ChangeLog Entry
0	2016-01-14 01:47:43	ID requested
1	2016-04-18 08:39:18	announced
⏵ 2	2024-10-22 04:27:27	announced	2024-10-22: Updated project metadata.

Zhu G, Sun L, Albanetti T, Linkous T, Larkin C, Schoner R, McGivney JB, Dovichi NJ, Quantitative analysis of the supernatant from host and transfected CHO cells using iTRAQ 8-plex technique. Biotechnol Bioeng, 113(10):2140-8(2016) [pubmed]

10.1002/bit.25991;

curator keyword: Biological

submitter keyword: CHO supernatant, iTRAQ 8-PLEX, LC-MS/MS

Norman Dovichi
contact affiliation	University of Notre Dame
contact email	ndovichi@nd.edu
lab head
Guijie Zhu
contact affiliation	University of Notre Dame
contact email	gzhu@nd.edu
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2016/07/PXD003462

PRIDE project URI

• PRIDE
  1. PXD003462
     1. Label: PRIDE project
     2. Name: Quantitative analysis of the supernatant from native and transfected CHO cells using iTRAQ 8-plex technique