PXD003428-2

PXD003428 is an original dataset announced via ProteomeXchange.

Title	Succinylation proteome analysis of seedling leaves of Bd21
Description	Protein lysine succinylation, an emerging protein post-translational modification widespread among microbiology, animals and plants, represents an important regulator of cellular processes. In our study, we took the first succinylation proteome analysis in the seedling leaves of Bd21 to draw a schematic map. With high accuracy nano LC-MS/MS combined with affinity purification, a total of 605 succinylated peptides in 323 proteins were identified and were implicated in various molecular functions and cellular biological processes. More than half (53.4%) of the proteins only have one lysine succinylated site. These identified proteins are involved in a variety of cellular functions such as amino acids transport/metabolism, post-translational modification, translation/ribosomal structure and lipid metabolism, especially energy and carbohydrate metabolism via GO, conservation analysis, protein interaction network and other bioinformatics analysis. Motif-X analysis of the succinylation sites identified fourteen significantly enriched succinylation motifs (-Ksucc-----K-, -Ksucc------G- etc) for the first time in plants and will provide possible succinylation binding locus for future studies. Secondary structure analysis showed that the succinylation sites occurred predominantly in alpha helix and coil structures which similar with acetylation. 155 (59.2%) of the homologous succinylated proteins were also identified in other three species (E. coli, S. cerevisiae and H. sapiens). 119 (45.4%) succinylated proteins and 115 (19%) sites were also to be acetylated at the same time. Our succinylated protein data set provides a promising starting point for further functional analysis of succinylation in B. distachyon, which could facilitate the elucidation of the entire metabolic network in the model monocot plant.
HostingRepository	PRIDE
AnnounceDate	2024-10-22
AnnouncementXML	Submission_2024-10-22_04:24:13.418.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Shoumin Zhen
SpeciesList	scientific name: Brachypodium distachyon (Purple false brome) (Trachynia distachya); NCBI TaxID: 15368;
ModificationList	succinylated residue
Instrument	LTQ Orbitrap Elite

Revision	Datetime	Status	ChangeLog Entry
0	2016-01-06 06:30:34	ID requested
1	2018-10-26 05:54:50	announced
⏵ 2	2024-10-22 04:24:14	announced	2024-10-22: Updated project metadata.

Zhen S, Deng X, Wang J, Zhu G, Cao H, Yuan L, Yan Y, First Comprehensive Proteome Analyses of Lysine Acetylation and Succinylation in Seedling Leaves of Brachypodium distachyon L. Sci Rep, 6():31576(2016) [pubmed]

10.1038/srep31576;

curator keyword: Biological

submitter keyword: succinylation analysis seedling leaves of Bd21

Yueming Yan
contact affiliation	Laboratory of Molecular Genetics and Proteomics, College of Life Science, Capital Normal University, Beijing, China.
contact email	yanym@cnu.edu.cn
lab head
Shoumin Zhen
contact affiliation	Capital normal university
contact email	zhenshoumin2008@163.com
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD003428
     1. Label: PRIDE project
     2. Name: Succinylation proteome analysis of seedling leaves of Bd21