PXD003352-1

PXD003352 is an original dataset announced via ProteomeXchange.

Title	Tandem affinity purification and Mass spectrometry (TAP-MS) analysis of PCH1’s associated proteins in various Arabidopsis genetic backgrounds.
Description	Here we describe a series TAP-MS analyses in various mutant backgrounds of Arabidopsis thaliana to co-purify any PCH1’s associated proteins, using tagged PCH1 as bait (PCH1-His6-Flag3, either overexpressed or under the control of its own promoter). By combining TAP-MS with genetics, we investigate the in-vivo dependence of several proteins for maintaining the association of PCH1 with its interacting proteins. Our results refine the protein-protein interaction network of an existing interactome containing phytochromes, the COP1-SPAs complex and the ELF3-ELF4-LUX complex.
HostingRepository	PRIDE
AnnounceDate	2016-02-09
AnnouncementXML	Submission_2016-02-09_08:39:14.xml
DigitalObjectIdentifier	https://dx.doi.org/10.6019/PXD003352
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Supported dataset by repository
PrimarySubmitter	Sophe Alvarez
SpeciesList	scientific name: Arabidopsis thaliana (Mouse-ear cress); NCBI TaxID: 3702;
ModificationList	Oxidation; Carbamidomethyl; Deamidated
Instrument	LTQ Orbitrap Velos

Revision	Datetime	Status	ChangeLog Entry
0	2015-12-15 06:43:08	ID requested
⏵ 1	2016-02-09 08:39:15	announced

Huang H, Yoo CY, Bindbeutel R, Goldsworthy J, Tielking A, Alvarez S, Naldrett MJ, Evans BS, Chen M, Nusinow DA, PCH1 integrates circadian and light-signaling pathways to control photoperiod-responsive growth in Arabidopsis. Elife, 5():e13292(2016) [pubmed]

curator keyword: Biological

submitter keyword: Tandem AP-MS, circadian clock, Arabidopsis

Meter Nusinow
contact affiliation	Donald Danforth Plant Science Center
contact email	dnusinow@danforthcenter.org
lab head
Sophe Alvarez
contact affiliation	University of Nebraska - Lincoln
contact email	salvarez@unl.edu
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2016/02/PXD003352

PRIDE project URI

• PRIDE
  1. PXD003352
     1. Label: PRIDE project
     2. Name: Tandem affinity purification and Mass spectrometry (TAP-MS) analysis of PCH1’s associated proteins in various Arabidopsis genetic backgrounds.