PXD003348 is an
original dataset announced via ProteomeXchange.
Dataset Summary
Title | Quantification of sulfenic acid on model protein |
Description | Aldolase contains eight free cysteine residues without disulfide formation in its native state, which makes it a suitable model to manipulate the oxidative modifications on cysteine. H2O2 was chosen to treat aldolase since the principle product between which is sulfenic acid.To assess the reduction of the newly formed sulfenic acids on aldolase by arsenite, a differential alkylation strategy was adapted.To further investigate the formation of other reversible cysteine oxidations such as disulfide on aldolase, arsenite was replaced by DTT in the differential alkylation method to reduce all the reversible cysteine oxidations.To further validate the selectivity of the reducing ability of arsenite on sulfenic acid but not other oxidative modifications especially disulfide, lysozyme was employed in the differential alkylation method. Lysozyme has 8 cysteine residues all bound into disulfide bonds, thereby was not treated with H2O2 to avoid further oxidation. |
HostingRepository | PRIDE |
AnnounceDate | 2016-10-10 |
AnnouncementXML | Submission_2016-10-10_03:36:18.xml |
DigitalObjectIdentifier | https://dx.doi.org/10.6019/PXD003348 |
ReviewLevel | Peer-reviewed dataset |
DatasetOrigin | Original dataset |
RepositorySupport | Supported dataset by repository |
PrimarySubmitter | Ru Li |
SpeciesList | scientific name: Mammalia; NCBI TaxID: 40674; scientific name: Gallus gallus (Chicken); NCBI TaxID: 9031; scientific name: Lagomorpha; NCBI TaxID: 9975; |
ModificationList | N-ethylmaleimide derivatized cysteine; monohydroxylated residue; iodoacetamide derivatized residue |
Instrument | LTQ Orbitrap Velos |
Dataset History
Revision | Datetime | Status | ChangeLog Entry |
0 | 2015-12-15 02:28:47 | ID requested | |
⏵ 1 | 2016-10-10 03:36:19 | announced | |
Publication List
Li R, Klockenbusch C, Lin L, Jiang H, Lin S, Kast J, on Monocytes via NADPH Oxidase. J Proteome Res, 15(12):4221-4233(2016) [pubmed] |
Keyword List
curator keyword: Biological |
submitter keyword: sulfenic acid, aldolase, lysozyme, arsenite |
Contact List
Juergen Kast |
contact affiliation | Professor, University of British Columbia |
contact email | kast@brc.ubc.ca |
lab head | |
Ru Li |
contact affiliation | Univerisity of British Columbia |
contact email | rli@brc.ubc.ca |
dataset submitter | |
Full Dataset Link List
Dataset FTP location
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PRIDE project URI |
Repository Record List
[ + ]
[ - ]
- PRIDE
- PXD003348
- Label: PRIDE project
- Name: Quantification of sulfenic acid on model protein