PXD003287-1

PXD003287 is an original dataset announced via ProteomeXchange.

Title	ib101206
Description	P2 is a spontaneous mutant selected from P1, an entomopathogenic fungus Beauveria bassiana that hyper-produces extracellular protease(s), compared to P1. The major expressed serine protease, called SBP, is shown to be up-regulated at the transcriptional level in the P2 mutant strain. The insecticidal potential of P1 and P2 strains was evaluated against Tuta absoluta larvae under laboratory conditions. Both strains are effective but P2 showed stronger effect than P1. Median lethal concentration (LC50) of P2 is 26 fold lower than that of P1. Median lethal times (LT50) of P1 and P2 are 4.38 and 0.84 days using 105 conidia ml-1. Enzymatic assays analysis showed that extracellular enzymes are differently expressed by the two strains, especially proteases and chitinases. Two-dimensional gel electrophoresis and mass spectrometry analysis confirmed the overexpression by P2 strain of protease and chitinase and revealed further the over-expression of several protease isoforms. Here we show a direct link between fungal enzymatic profile and insect’s pathogenesis, giving a molecular explanation for the insecticidal potential of B. bassiana fungus.
HostingRepository	PRIDE
AnnounceDate	2018-03-19
AnnouncementXML	Submission_2018-03-19_09:54:05.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Dupuy Jean-William
SpeciesList	scientific name: Beauveria bassiana (White muscardine disease fungus) (Tritirachium shiotae); NCBI TaxID: 176275;
ModificationList	monohydroxylated residue; iodoacetamide derivatized residue
Instrument	LCQ Classic

Revision	Datetime	Status	ChangeLog Entry
0	2015-12-07 02:08:00	ID requested
⏵ 1	2018-03-19 09:54:05	announced
2	2020-03-12 03:39:05	announced	2020-03-12: Updated project metadata.
3	2023-11-14 06:40:27	announced	2023-11-14: Updated project metadata.

10.1007/s13593-016-0394-6;

curator keyword: Biological

submitter keyword: Beauvaria bassiana, LC-MS

Ali gargouri
contact affiliation	Laboratoire de Valorisation de la Biomasse et de Production de Protéines chez les Eucaryotes, Centre de Biotechnologie de Sfax, BP1177, 3018 Sfax-Tunisia. University of Sfax
contact email	faouzi.gargouri@cbs.rnrt.tn
lab head
Dupuy Jean-William
contact affiliation	Plateforme Proteome Bordeaux
contact email	jean-william.dupuy@u-bordeaux.fr
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2018/03/PXD003287

PRIDE project URI

• PRIDE
  1. PXD003287
     1. Label: PRIDE project
     2. Name: ib101206