PXD003286-2

PXD003286 is an original dataset announced via ProteomeXchange.

Title	Changes in Gab2 phosphorylation and interaction partners in response to interleukin (IL)-2 stimulation in T-lymphocytes
Description	Interleukin-2 (IL-2) stimulation results in T-cell growth as a consequence of activation of highly sophisticated and fine-tuned signaling pathways. Despite lacking intrinsic enzymatic activity, scaffold proteins such as Gab2, play a pivotal role in IL-2-triggered signal transduction integrating, diversifying and amplifying the signal by serving as a platform for the assembly of effectors proteins. Traditionally, Gab2-mediated protein recruitment was believed to solely depend on cytokine-induced phosphotyrosine moieties. At present, increasing body of literature indicates that phosphorylation on serine/threonine residues are emerging also as key mediators of Gab2-dependent signal regulation. Despite its relevance, IL-2-triggered regulation on Gab2 phosphorylation is yet poorly understood. Combining antibody- and TiO2-based enrichment of Gab2 with SILAC quantitative mass spectrometry we disclose the prominent regulation on serine/threonine phosphorylated residues of Gab2 by IL-2 showing that at least 18 serines and 1 threonine, including previously non-reported ones, become phosphorylated in response to the cytokine. Additionally, we deciphered the interactome of the scaffold protein in resting and cytokine-treated T-lymphocytes and besides well-known Gab2 interactors we uncover three novel cytokine-inducible Gab2-binding proteins. Thus, our data provide novel insights and a wealth of candidates for future studies that will shed light into the role of Gab2 in IL-2-initiated signal transduction.
HostingRepository	PRIDE
AnnounceDate	2024-10-22
AnnouncementXML	Submission_2024-10-22_04:27:02.060.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Nerea Osinalde
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	phosphorylated residue
Instrument	Q Exactive

Revision	Datetime	Status	ChangeLog Entry
0	2015-12-07 02:02:12	ID requested
1	2016-04-11 01:41:58	announced
⏵ 2	2024-10-22 04:27:05	announced	2024-10-22: Updated project metadata.

Osinalde N, S, á, nchez-Quiles V, Blagoev B, Kratchmarova I, Changes in Gab2 phosphorylation and interaction partners in response to interleukin (IL)-2 stimulation in T-lymphocytes. Sci Rep, 6():23530(2016) [pubmed]

10.1038/srep23530;

curator keyword: Biological, Biomedical

submitter keyword: scaffold protein, T-lymphocytes, phosphorylation,IL-2, GAB2, interactome

Irina kratchmarova
contact affiliation	Department of Biochemistry and Molecular Biology, University of Southern Denmark, 5230 Odense M, Denmark.
contact email	ihk@bmb.sdu.dk
lab head
Nerea Osinalde
contact affiliation	Postdoc
contact email	nmo@bmb.sdu.dk
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD003286
     1. Label: PRIDE project
     2. Name: Changes in Gab2 phosphorylation and interaction partners in response to interleukin (IL)-2 stimulation in T-lymphocytes