PXD003275-1

PXD003275 is an original dataset announced via ProteomeXchange.

Title	Differential proteomics of Lenisia limosa (Breviatea) and its epibiotic Arcobacter symbiont
Description	The Breviatea form a lineage of free-living protists that emerged over 800 million years ago as a sister clade to opistokonta, comprising animals and fungi. Breviates conserved the ability to thrive in absence of oxygen which was an important adaptation to the low oceanic oxygen-levels that prevailed by that time. We previously found that the novel breviate, Lenisia limosa, gen. et sp. nov., was opportunistically colonized by relatives of animal-associated Arcobacter. Here we used differential proteomics to investigate how the presence/absence of symbiotic Arcobacter is manifested in Lenisia limosa's proteome. Vice versa, we also measured how symbiosis is reflected in Arcobacter's proteome. The results provide a resource to characterize the molecular underpinnings of a novel protist-prokaryote symbiosis.
HostingRepository	PRIDE
AnnounceDate	2016-05-25
AnnouncementXML	Submission_2016-05-25_08:39:19.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Manuel Kleiner
SpeciesList	scientific name: Breviatea; NCBI TaxID: 1401294; scientific name: Arcobacter; NCBI TaxID: 28196; scientific name: Alteromonas macleodii ATCC 27126; NCBI TaxID: 529120;
ModificationList	monohydroxylated residue; acetylated residue; iodoacetamide derivatized residue
Instrument	Q Exactive

Revision	Datetime	Status	ChangeLog Entry
0	2015-12-03 02:59:02	ID requested
⏵ 1	2016-05-25 08:39:20	announced
2	2016-07-04 05:02:07	announced	Updated publication reference for PubMed record(s): 27279223.
3	2024-10-22 04:21:05	announced	2024-10-22: Updated project metadata.

Dataset with its publication pending

curator keyword: Biological

submitter keyword: Lenisia limosa, Q Exactive plus, LC-MS/MS, Easy nLC 1000, symbiosis, metaproteomics, microbial community

Marc Strous
contact affiliation	University of Calgary, Department of Geoscience
contact email	mstrous@ucalgary.ca
lab head
Manuel Kleiner
contact affiliation	University of Calgary
contact email	manuel.kleiner@ucalgary.ca
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2016/05/PXD003275

PRIDE project URI

• PRIDE
  1. PXD003275
     1. Label: PRIDE project
     2. Name: Differential proteomics of Lenisia limosa (Breviatea) and its epibiotic Arcobacter symbiont