PXD003215-1

PXD003215 is an original dataset announced via ProteomeXchange.

Title	A Novel Method for Isolating Whole Protein from Human Cranial Bone
Description	The presence of the extracellular matrix within human bone limits the applicability of conventional protocols for protein extraction. As a result, complete and accurate characterization of human bone proteome is yet to be performed, and as a result, several bone-related diseases such as craniosynostosis and osteosarcoma are still poorly understood. We sought to develop a reproducible method for extracting whole proteins of varying molecular weights from human cranial bone. Whole protein was extracted from human cranial suture by mechanically processing samples using a method that minimized heat introduction to proteins to limit degradation. Western blotting suggests the presence of whole protein while mass spectrometry was used to sequence peptides and identify isolated proteins. Molecular weights of extracted protein ranged from 9.4-629 kDa and contained proteins of both intra- and extra-cellular origin. High correlation scores among suture protein spectral counts suggest the reproducibility of the method. Ontology analytics revealed proteins of myriad functions including mediators of metabolic processes and cell organelles. These results suggest a reproducible method for isolation of whole protein representing a large range of molecular weights, origins and functions.
HostingRepository	PRIDE
AnnounceDate	2016-10-05
AnnouncementXML	Submission_2016-10-05_04:27:52.xml
DigitalObjectIdentifier	https://dx.doi.org/10.6019/PXD003215
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Supported dataset by repository
PrimarySubmitter	Donald Wolfgeher
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	ubiquitination signature dipeptidyl lysine; phosphorylated residue; monohydroxylated residue; formylated residue; iodoacetamide derivatized residue
Instrument	Q Exactive

Revision	Datetime	Status	ChangeLog Entry
0	2015-11-19 01:51:11	ID requested
⏵ 1	2016-10-05 04:27:54	announced

Lyon SM, Mayampurath A, Rogers MR, Wolfgeher DJ, Fisher SM, Volchenboum SL, He TC, Reid RR, A method for whole protein isolation from human cranial bone. Anal Biochem, 515():33-39(2016) [pubmed]

curator keyword: Technical

submitter keyword: Q-Exactive, Protein, Extraction, Human, Method, Whole Protein, Cranial Bone, Proteome

Dr. Russell R. Reid, MD, PhD
contact affiliation	The Laboratory of Craniofacial Development and Biology, Section of Plastic and Reconstructive Surgery, University of Chicago Medicine
contact email	rreid@surgery.bsd.uchicago.edu
lab head
Donald Wolfgeher
contact affiliation	University of Chicago
contact email	donw@uchicago.edu
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD003215
     1. Label: PRIDE project
     2. Name: A Novel Method for Isolating Whole Protein from Human Cranial Bone