PXD003212-1

PXD003212 is an original dataset announced via ProteomeXchange.

Title	The Trichomonas PACK proteome
Description	Actin and tubulin are rather conserved and easy to trace throughout eukaryotic evolution, but less so proteins of the intermediate filament (IF) family. Canonical IF protein families appear restricted to Metazoa, although protists are well known for an extensive number of filamentous structures that neither involve actin nor tubulin, yet support the complex cell architecture. Trichomonadida are an evolutionary early branching group of excavate parasites that are united by a unique cytoskeletal framework, which includes the costa. This rod-like structure supports the flagellum that is attached to the undulating membrane. We determined the proteome of the detergent resistant cytoskeletal backbone of Tetratrichomonas gallinarum (the pelta axostyle costa karyomastigont complex, PACK) to identify the proteins that constitute these structures and to investigate their properties. Among the 582 proteins identified in each of the three individual proteome screens, we found major components of the costa whose association was verified through in vivo localization experiments. Intriguingly, many of the PACK proteins are characterized by recognizable features, including long central coiled-coil regions, that are reminiscent of metazoan IF proteins. The newly identified cytoskeletal proteins cannot be attributed to any known proteins of the metazoan IF families, albeit they resemble these in terms of their predicted tertiary structure and the more general function. Our data offer further support that IF proteins of protists, which form cytoskeletal filaments that do not depend on actin and tubulin, evolved many times independently in eukaryotes.
HostingRepository	PRIDE
AnnounceDate	2016-10-10
AnnouncementXML	Submission_2016-10-10_07:10:13.xml
DigitalObjectIdentifier	https://dx.doi.org/10.6019/PXD003212
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Supported dataset by repository
PrimarySubmitter	Gereon Poschmann
SpeciesList	scientific name: Tetratrichomonas gallinarum; NCBI TaxID: 5730;
ModificationList	Oxidation; Acetyl; Carbamidomethyl
Instrument	LTQ Orbitrap Elite

Revision	Datetime	Status	ChangeLog Entry
0	2015-11-19 01:03:09	ID requested
⏵ 1	2016-10-10 07:10:14	announced
2	2016-10-18 02:56:02	announced	Updated publication reference for PubMed record(s): 27744090.
3	2016-11-15 05:01:59	announced	Updated project metadata.
4	2017-10-24 01:53:44	announced	Updated project metadata.

Dataset with its publication pending

curator keyword: Biological

submitter keyword: LC-MS, Tetratrichomonas gallinarum, evolution, cytoskeleton

Gereon Poschmann
contact affiliation	Molecular Proteomics Laboratory Heinrich Heine Universität Universitätsstr. 1 40225 Düsseldorf
contact email	gereon.poschmann@hhu.de
lab head
Gereon Poschmann
contact affiliation	Heinrich Heine Universität Düsseldorf
contact email	gereon.poschmann@rub.de
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2016/10/PXD003212

PRIDE project URI

• PRIDE
  1. PXD003212
     1. Label: PRIDE project
     2. Name: The Trichomonas PACK proteome