PXD003209-2

PXD003209 is an original dataset announced via ProteomeXchange.

Title	Proteomics of Extracellular Vesicles Harvested with ExtraPEG
Description	Polyethylene glycol (PEG) is commonly used to isolate virus particles from culture medium. This project adapted and optimized the virus procedure for harvesting extracellular vesicles (of similar biophysical properties to viruses) from media. An 8% PEG solution harvested the purest vesicles. Followed by a wash step (ultracentrifugation of concentrated vesicles in saline), this method produced preparations comparable or superior to commonly used methods in terms of purity, abundance, and cost. It is also highly adaptable, allowing the procedure to be used for enriching vesicles from a variety of biological fluids. As proof of principle, extracellular vesicle protein was successfully harvested from HeLa cell medium using the described PEG-based harvest method and analyzed by mass spectrophotometry.
HostingRepository	PRIDE
AnnounceDate	2016-04-18
AnnouncementXML	Submission_2016-04-18_07:01:20.xml
DigitalObjectIdentifier	https://dx.doi.org/10.6019/PXD003209
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Supported dataset by repository
PrimarySubmitter	Mark Rider
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	Oxidation; Phospho; Dehydrated; Ammonia-loss; Acetyl; Carbamidomethyl
Instrument	LTQ Orbitrap Velos

Revision	Datetime	Status	ChangeLog Entry
0	2015-11-18 05:26:22	ID requested
1	2016-04-18 06:48:43	announced
⏵ 2	2016-04-18 07:01:21	announced	Updated publication reference for PubMed record(s): 27068479.

Rider MA, Hurwitz SN, Meckes DG, ExtraPEG: A Polyethylene Glycol-Based Method for Enrichment of Extracellular Vesicles. Sci Rep, 6():23978(2016) [pubmed]

submitter keyword: Polyethylene glycol, extracellular vesicle, exosome

David G. Meckes Jr.
contact affiliation	Department of Biomedical Sciences, Florida State University College of Medicine, USA
contact email	David.Meckes@med.fsu.edu
lab head
Mark Rider
contact affiliation	Florida State University
contact email	markarider@gmail.com
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2016/04/PXD003209

PRIDE project URI

• PRIDE
  1. PXD003209
     1. Label: PRIDE project
     2. Name: Proteomics of Extracellular Vesicles Harvested with ExtraPEG