PXD003198-1

PXD003198 is an original dataset announced via ProteomeXchange.

Title	Characterisation of pancreatic ductal adenocarcinoma subtypes by global phosphotyrosine profiling
Description	Here we use high-resolution mass spectrometry (MS) to characterise the magnitude and complexity of global tyrosine phosphorylation PDAC. We profiled the endogenous tyrosine phosphorylation-based signaling in 36 cell lines from two cell line populations, quantifying around 2,000 tyrosine phosphorylation sites. This approach facilitated consistent segregation of PDAC cell lines into three subtypes with distinct signalling profiles, using both their global tyrosine phosphorylation patterns and a Random Forest derived 8 phosphorylation site signature. We extracted segregation-driving phosphosites, and subsequent pathway and network analysis to characterise the identified subtypes.
HostingRepository	PRIDE
AnnounceDate	2016-06-09
AnnouncementXML	Submission_2016-06-09_07:16:09.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Emily Humphrey
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	phosphorylated residue
Instrument	Q Exactive; LTQ Orbitrap Velos

Revision	Datetime	Status	ChangeLog Entry
0	2015-11-16 08:24:54	ID requested
⏵ 1	2016-06-09 07:16:10	announced
2	2024-10-22 04:29:11	announced	2024-10-22: Updated project metadata.

Humphrey ES, Su SP, Nagrial AM, Hochgr, ä, fe F, Pajic M, Lehrbach GM, Parton RG, Yap AS, Horvath LG, Chang DK, Biankin AV, Wu J, Daly RJ, Resolution of Novel Pancreatic Ductal Adenocarcinoma Subtypes by Global Phosphotyrosine Profiling. Mol Cell Proteomics, 15(8):2671-85(2016) [pubmed]

submitter keyword: Pancreatic cancer, phosphotyrosine, phosphoproteomics, phosphoproteome, shotgun, subtypes

Professor Roger Daly
contact affiliation	Head, Department of Biochemistry and Molecular Biology, Monash University Head, Signalling Network Laboratory, Monash University Head, Biomedicine Discovery Institute Cancer Program, Monash University
contact email	roger.daly@monash.edu
lab head
Emily Humphrey
contact affiliation	Max Planck Institute of Biochemistry
contact email	emily.s.humphrey@gmail.com
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2016/06/PXD003198

PRIDE project URI

• PRIDE
  1. PXD003198
     1. Label: PRIDE project
     2. Name: Characterisation of pancreatic ductal adenocarcinoma subtypes by global phosphotyrosine profiling