PXD003144-1

PXD003144 is an original dataset announced via ProteomeXchange.

Title	Measuring C2C12 Myotube Protein Expression Changes in Inflammation using Punch-P
Description	Selective identification of newly synthesized proteins provides insight on biological processes that depend on the continued synthesis of specific proteins to maintain homeostasis as well as those that are up- or down-regulated in response to changing needs. We used puromycin-associated nascent chain proteomics (PUNCH-P) to characterize new protein synthesis in C2C12 myotubes and changes resulting from exposure to a combination of lipopolysaccharide and interferon-(LPS/IFN) for either a short (4h) or prolonged (16h) time period. We identified sequences of nascent polypeptide chains belonging to a total of 1,523 proteins and report their detection within three independent samples for each growth condition. The identified proteins correspond to approximately 15% of presently known proteins in C2C12 myotubes and are enriched in specific cellular components and pathways. A subset of these identified proteins have functional characteristics and were identified only in treated samples, consistent with new protein synthesis in response to LPS/IFN. Thus, the identification of proteins from their nascent polypeptide chains provides a resource to analyze the role of newly synthesizing proteins in protein homeostasis and proteome responses in C2C12 myotubes.
HostingRepository	PRIDE
AnnounceDate	2022-02-28
AnnouncementXML	Submission_2022-02-28_02:43:47.983.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Bruce Stanley
SpeciesList	scientific name: Mus musculus (Mouse); NCBI TaxID: 10090;
ModificationList	S-carboxamidomethyl-L-cysteine
Instrument	TripleTOF 5600

Revision	Datetime	Status	ChangeLog Entry
0	2015-11-04 01:37:34	ID requested
⏵ 1	2022-02-28 02:43:49	announced
2	2024-10-22 05:34:06	announced	2024-10-22: Updated project metadata.

Coleman CS, Stanley BA, Lang CH, . Inflammation, 45(3):1313-1331(2022) [pubmed]

curator keyword: Biomedical

submitter keyword: mouse, muscle, 5600 TripleTOF, myotubes, LPS, interferon gamma, inflammatio

Bruce A. Stanley
contact affiliation	Section of Research Resources H093 The Pennsylvania State University College of Medicine 500 University Drive Hershey, PA 17033-0850 USA
contact email	bstanley@psu.edu
lab head
Bruce Stanley
contact affiliation	Penn State College of Medicine
contact email	bstanley@psu.edu
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD003144
     1. Label: PRIDE project
     2. Name: Measuring C2C12 Myotube Protein Expression Changes in Inflammation using Punch-P