PXD003049 is an
original dataset announced via ProteomeXchange.
Dataset Summary
Title | Identification of calredoxin (C. reinhardtii) interaction partners by affinity chromatography and LC-MS/MS |
Description | Calcium (Ca2+) and redox signaling play important roles in acclimation processes from archaea to eukaryotic organisms. Herein we characterized a unique protein from Chlamydomonas reinhardtii that has the competence to integrate Ca2+- and redox-related signaling. This protein, designated as calredoxin (CRX), combines four Ca2+-binding EF-hands and a thioredoxin (TRX) domain. A crystal structure of CRX, at 1.6 Å resolution, revealed an unusual calmodulin-fold of the Ca2+-binding EF-hands, which is functionally linked via an inter-domain communication path with the enzymatically active TRX domain. CRX is chloroplast-localized and interacted with a chloroplast 2-Cys-peroxiredoxin (PRX1). Ca2+-binding to CRX is critical for its TRX activity and for efficient binding and reduction of PRX1. Thereby, CRX represents a new class of Ca2+-dependent "sensor-responder" proteins. Genetically engineered Chlamydomonas strains with strongly diminished amounts of CRX revealed altered photosynthetic electron transfer and were affected in oxidative stress response underpinning a function of CRX in stress acclimation. |
HostingRepository | PRIDE |
AnnounceDate | 2016-10-12 |
AnnouncementXML | Submission_2016-10-12_03:46:58.xml |
DigitalObjectIdentifier | |
ReviewLevel | Peer-reviewed dataset |
DatasetOrigin | Original dataset |
RepositorySupport | Unsupported dataset by repository |
PrimarySubmitter | Martin Scholz |
SpeciesList | scientific name: Chlamydomonas reinhardtii; NCBI TaxID: 3055; |
ModificationList | phosphorylated residue; monohydroxylated residue; acetylated residue; iodoacetamide derivatized residue |
Instrument | Q Exactive |
Dataset History
Revision | Datetime | Status | ChangeLog Entry |
0 | 2015-10-13 07:23:14 | ID requested | |
⏵ 1 | 2016-10-12 03:46:59 | announced | |
Publication List
Hochmal AK, Zinzius K, Charoenwattanasatien R, G, ä, belein P, Mutoh R, Tanaka H, Schulze S, Liu G, Scholz M, Nordhues A, Offenborn JN, Petroutsos D, Finazzi G, Fufezan C, Huang K, Kurisu G, Hippler M, Calredoxin represents a novel type of calcium-dependent sensor-responder connected to redox regulation in the chloroplast. Nat Commun, 7():11847(2016) [pubmed] |
Keyword List
curator keyword: Biological |
submitter keyword: Chlamydomonas, calredoxin |
Contact List
Michael Hippler |
contact affiliation | WWU Muenster, Institute of Plant Biology and Biotechnology |
contact email | mhippler@uni-muenster.de |
lab head | |
Martin Scholz |
contact affiliation | IBBP Muenster |
contact email | mscholz@uni-muenster.de |
dataset submitter | |
Full Dataset Link List
Dataset FTP location
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PRIDE project URI |
Repository Record List
[ + ]
[ - ]
- PRIDE
- PXD003049
- Label: PRIDE project
- Name: Identification of calredoxin (C. reinhardtii) interaction partners by affinity chromatography and LC-MS/MS