PXD003043 is an
original dataset announced via ProteomeXchange.
Dataset Summary
Title | mouse liver microsomes phosphorylation with LC-MS/MS |
Description | The microsomes are re-formed vesicle-like artifacts, not ordinarily present in living cells, from pieces of the endoplasmic reticulum (ER), plasma membrane, mitochondria, and Golgi apparatus of broken eukaryotic cells. Typically, microsomes are separated by differential centrifuged from homogenized of cell or tissue. Based on enrichment of ER fractions, prepared microsomes mainly show a large of metabolic activity following high amount of metabolic enzyme, as cytochrome P450 (CYP). CYPs are important components of xenobiotic-metabolizing monooxygenase accounting for an approximate 75% of biotransformation of clinically relevant drugs. Hepatic CYP are ER-anchored hemoproteins involved in the metabolism of numerous endo- and xenobiotics including drugs, steroids, and carcinogens. The CYPs catalytic activity is subject to regulation via phosphorylation. In several previous studies, 8 CYP phosphorylation sites had been discovered in human. Protein kinase A (PKA) and protein kinase C (PKC) were identified as a major catalyst for the phosphorylation of CYPs in 2003. The phosphorylation of CYPs process to their ubiquitination undergoing ubiquitin-dependent 26S proteosomal degradation. |
HostingRepository | PRIDE |
AnnounceDate | 2017-04-04 |
AnnouncementXML | Submission_2017-04-04_02:14:22.xml |
DigitalObjectIdentifier | |
ReviewLevel | Peer-reviewed dataset |
DatasetOrigin | Original dataset |
RepositorySupport | Unsupported dataset by repository |
PrimarySubmitter | Oh Kwang Kwon |
SpeciesList | scientific name: Mus musculus (Mouse); NCBI TaxID: 10090; |
ModificationList | phosphorylated residue |
Instrument | LTQ Orbitrap Elite |
Dataset History
Revision | Datetime | Status | ChangeLog Entry |
0 | 2015-10-13 01:32:20 | ID requested | |
⏵ 1 | 2017-04-04 02:14:23 | announced | |
Publication List
Kwon OK, Sim J, Kim SJ, Sung E, Kim JY, Jeong TC, Lee S, Comprehensive Analysis of in Vivo Phosphoproteome of Mouse Liver Microsomes. J Proteome Res, 14(12):5215-24(2015) [pubmed] |
Keyword List
curator keyword: Biological |
submitter keyword: mouse liver microsome, phosphorylation, TiO2, LC-MS/MS |
Contact List
Sangkyu Lee |
contact affiliation | College of Pharmacy, Research Institute of Pharmaceutical Sciences, Kyungpook National University |
contact email | sangkyu@knu.ac.kr |
lab head | |
Oh Kwang Kwon |
contact affiliation | Kyungpook National University |
contact email | kok0100@nate.com |
dataset submitter | |
Full Dataset Link List
Dataset FTP location
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PRIDE project URI |
Repository Record List
[ + ]
[ - ]
- PRIDE
- PXD003043
- Label: PRIDE project
- Name: mouse liver microsomes phosphorylation with LC-MS/MS