PXD003031 is an
original dataset announced via ProteomeXchange.
Dataset Summary
Title | Phosphorylation of Asc1p and Asc1p-dependent phospho-proteome |
Description | Phosphorylation of Asc1p and Asc1p-dependent phospho-proteome We determined 1) the phosphorylation sites within the Asc1 protein purified from Saccharomyces cerevisiae via its Strep-tag and 2) the Asc1p-dependent phospho-proteome. 1) Phospho-sites within tryptic peptides of Asc1p were identified using the Proteome Discoverer 1.4 software with the SequestHT and Mascot search engines and phosphorylation site localization was evaluated with the phosphoRS tool. 2) Quantitative triple SILAC-based phospho-proteome comparison of an ASC1 wild-type strain with asc1 mutant strains. MS data were analyzed with MaxQuant and Perseus. |
HostingRepository | PRIDE |
AnnounceDate | 2017-10-24 |
AnnouncementXML | Submission_2017-10-24_02:41:37.xml |
DigitalObjectIdentifier | |
ReviewLevel | Peer-reviewed dataset |
DatasetOrigin | Original dataset |
RepositorySupport | Unsupported dataset by repository |
PrimarySubmitter | Oliver Valerius |
SpeciesList | scientific name: Saccharomyces cerevisiae (Baker's yeast); NCBI TaxID: 4932; |
ModificationList | phosphorylated residue; isotope labeled residue; monohydroxylated residue; iodoacetamide derivatized residue |
Instrument | LTQ Orbitrap Velos |
Dataset History
Revision | Datetime | Status | ChangeLog Entry |
0 | 2015-10-12 00:57:16 | ID requested | |
1 | 2016-12-13 08:14:26 | announced | |
⏵ 2 | 2017-10-24 02:41:38 | announced | Updated project metadata. |
Publication List
Schmitt K, Smolinski N, Neumann P, Schmaul S, Hofer-Pretz V, Braus GH, Valerius O, Asc1p/RACK1 Connects Ribosomes to Eukaryotic Phosphosignaling. Mol Cell Biol, 37(3):(2017) [pubmed] |
Keyword List
curator keyword: Biological |
submitter keyword: Saccharomyces cerevisiae, Asc1p, RACK1, LC-MSMS, SILAC, signal transduction, translation, phosphorylation |
Contact List
Dr. Oliver Valerius |
contact affiliation | Georg-August-University Göttingen Department of Molecular Microbiology and Genetics Institute of Microbiology and Genetics |
contact email | ovaleri@gwdg.de |
lab head | |
Oliver Valerius |
contact affiliation | Georg-August-Universität Göttingen |
contact email | ovaleri@gwdg.de |
dataset submitter | |
Full Dataset Link List
Dataset FTP location
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PRIDE project URI |
Repository Record List
[ + ]
[ - ]
- PRIDE
- PXD003031
- Label: PRIDE project
- Name: Phosphorylation of Asc1p and Asc1p-dependent phospho-proteome