PXD003022 is an
original dataset announced via ProteomeXchange.
Dataset Summary
Title | Tetranychus urticae secretome |
Description | The two-spotted spider mite Tetranychus urticae is an extreme polyphaguous crop pest. Next to an increased detoxification potential of plant secondary metabolites, it has recently been shown that spider mites manipulate plant defences. Salivary constituents are proposed to play an important role during the interaction with its many hosts. The proteomic composition of saliva delivered into artificial diet by spider mites adapted to various hosts - bean, soy, maize, tomato -was determined using Orbitrap mass spectrometry. Over 200 different proteins were identified, many of unknown function and in numerous cases belonging to multi-membered gene families. A selection of these putative salivary proteins was validated using whole-mount in situ hybridizations and expression was shown to be localized in the anterior and dorsal podocephalic glands of the spider mite. Host-plant dependent expression was evident from the proteomics dataset and was further studied in detail by micro-array based genome wide gene expression profiling of mites maintained on the host plants under study. Previously obtained gene-expression datasets were further used to get more insight in the expression profile over different life stages and physiological states. To conclude, for the first time the T. urticae salivary proteome repertoire was characterized using a custom feeding hemisphere-based enrichment technique. This knowledge will assist in unraveling the molecular interactions between phytophagous mites and their host plants. This may ultimately facilitate the development of mite-resistant crops. |
HostingRepository | PRIDE |
AnnounceDate | 2017-01-17 |
AnnouncementXML | Submission_2017-02-06_01:13:55.xml |
DigitalObjectIdentifier | https://dx.doi.org/10.6019/PXD003022 |
ReviewLevel | Peer-reviewed dataset |
DatasetOrigin | Original dataset |
RepositorySupport | Supported dataset by repository |
PrimarySubmitter | Gerben Menschaert |
SpeciesList | scientific name: Tetranychus urticae (Two-spotted spider mite); NCBI TaxID: 32264; |
ModificationList | No PTMs are included in the dataset |
Instrument | LTQ Orbitrap |
Dataset History
Revision | Datetime | Status | ChangeLog Entry |
0 | 2015-10-08 02:53:37 | ID requested | |
1 | 2017-01-17 03:04:10 | announced | |
⏵ 2 | 2017-02-06 01:13:57 | announced | Updated publication reference for PubMed record(s): 27703040. |
Publication List
Jonckheere W, Dermauw W, Zhurov V, Wybouw N, Van den Bulcke J, Villarroel CA, Greenhalgh R, Grbi, ć M, Schuurink RC, Tirry L, Baggerman G, Clark RM, Kant MR, Vanholme B, Menschaert G, Van Leeuwen T, The Salivary Protein Repertoire of the Polyphagous Spider Mite Tetranychus urticae: A Quest for Effectors. Mol Cell Proteomics, 15(12):3594-3613(2016) [pubmed] |
Keyword List
curator keyword: Biological |
submitter keyword: Tetranichus urticae, secretome, |
Contact List
Gerben Menschaert |
contact affiliation | BioBix, Lab for Bioinformatics and Computational Genomics, Ghent University, Belgium |
contact email | Gerben.Menschaert@gmail.com |
lab head | |
Gerben Menschaert |
contact affiliation | Faculty of Bioscience Engineering |
contact email | gerben.menschaert@gmail.com |
dataset submitter | |
Full Dataset Link List
Dataset FTP location
NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2017/01/PXD003022 |
PRIDE project URI |
Repository Record List
[ + ]
[ - ]
- PRIDE
- PXD003022
- Label: PRIDE project
- Name: Tetranychus urticae secretome