PXD003005 is an
original dataset announced via ProteomeXchange.
Dataset Summary
Title | Mouse wild-type and Anxa5-null utricles and hair bundles |
Description | The phospholipid- and Ca2+-binding protein annexin A5 (ANXA5) is the most abundant membrane-associated protein of ~P23 mouse vestibular hair bundles, present at ~15,000 copies per stereocilium, which is ~2% of the total protein there. Although seven other annexin genes are expressed in mouse utricles, mass spectrometry showed that none were upregulated in stereocilia of Anxa5-/- mice. Annexins have been proposed to mediate Ca2+-dependent repair of membrane lesions, which could be part of the repair mechanism in hair cells after noise damage. Nevertheless, Anxa5-/- mice not only have normal hearing or balance, but are identical to wild-type mice in their temporary or permanent hearing changes following noise exposure. We suggest that the significant amounts of ANXA2 and ANXA6 that are present in the hair-cell cytoplasm can substitute in any function carried out by ANXA5, and that ANXA5 may be playing a hitchhiker role in the bundle. These results demonstrate that abundance of a protein in a specific compartment or cellular structure does not necessarily imply functional significance. |
HostingRepository | PRIDE |
AnnounceDate | 2016-06-09 |
AnnouncementXML | Submission_2016-06-09_07:56:51.xml |
DigitalObjectIdentifier | |
ReviewLevel | Peer-reviewed dataset |
DatasetOrigin | Original dataset |
RepositorySupport | Unsupported dataset by repository |
PrimarySubmitter | Peter Barr-Gillespie |
SpeciesList | scientific name: Mus musculus (Mouse); NCBI TaxID: 10090; |
ModificationList | iodoacetamide derivatized residue |
Instrument | LTQ Velos |
Dataset History
Revision | Datetime | Status | ChangeLog Entry |
0 | 2015-10-05 01:48:24 | ID requested | |
⏵ 1 | 2016-06-09 07:56:52 | announced | |
Publication List
Krey JF, Drummond M, Foster S, Porsov E, Vijayakumar S, Choi D, Friderici K, Jones SM, Nuttall AL, Barr-Gillespie PG, Annexin A5 is the Most Abundant Membrane-Associated Protein in Stereocilia but is Dispensable for Hair-Bundle Development and Function. Sci Rep, 6():27221(2016) [pubmed] |
Keyword List
submitter keyword: hair cells, stereocilia, annexins, vestibular |
Contact List
Peter Barr-Gillespie |
contact affiliation | Oregon Hearing Research Center Oregon Health & Science University Portland OR 97239 |
contact email | gillespp@ohsu.edu |
lab head | |
Peter Barr-Gillespie |
contact affiliation | Oregon Hearing Research Center |
contact email | gillespp@ohsu.edu |
dataset submitter | |
Full Dataset Link List
Dataset FTP location
NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2016/06/PXD003005 |
PRIDE project URI |
Repository Record List
[ + ]
[ - ]
- PRIDE
- PXD003005
- Label: PRIDE project
- Name: Mouse wild-type and Anxa5-null utricles and hair bundles