PXD002985 is an
original dataset announced via ProteomeXchange.
Dataset Summary
Title | Proteomic analysis revealed the important role of vimentin in human cervical carcinoma HeLa cells treated with gambogic acid |
Description | Gambogic acid (GA) is an anti-cancer agent in phase Ⅱb clinical trial in China. In HeLa cells, GA inhibited cell proliferation, induced cell cycle arrest at G2/M phase and apoptosis, as showed by results of MTT assay and flow cytometric analysis. Possible target-related proteins of GA were searched using comparative proteomic analysis (2-DE) and 9 proteins at early (3 h) stage together with 9 proteins at late (24 h) stage were found. Vimentin was the only target-related protein found at both early and late stage. Results of both 2-DE analysis and Western blotting assay suggested cleavage of vimentin induced by GA. MS/MS analysis of cleaved vimentin peptides indicated possible cleavage sites of vimentin at or near aa51 and aa425. Results of targeted proteomic analysis showed that GA induced change in phosphorylation state of the vimentin head domain (aa51-64). Caspase inhibitors could not abrogate GA-induced cleavage of vimentin. Over-expression of vimentin ameliorated cytotoxicity of GA in HeLa cells. The GA-activated signal transduction, from p38 MAPK, heat shock protein 27 (HSP27), vimentin, dysfunction of cytoskeleton, to cell death, was predicted and then confirmed. Results of animal study showed that GA treatment inhibited tumor growth in HeLa tumor-bearing mice and cleavage of vimentin could be observed in tumor xenografts of GA-treated animals. Results of immunohistochemical staining also showed down-regulated vimentin level in tumor xenografts of GA-treated animals. Furthermore, compared with cytotoxicity of GA in HeLa cells, cytotoxicity of GA in MCF-7 cells with low level of vimentin was weaker while cytotoxicity of GA in MG-63 cells with high level of vimentin was stronger. These results indicated the important role of vimentin in the cytotoxicity of GA. The effects of GA on vimentin and other epithelial-to-mesenchymal transition (EMT) markers provided suggestion for better usage of GA in clinic. |
HostingRepository | PRIDE |
AnnounceDate | 2018-10-24 |
AnnouncementXML | Submission_2018-10-24_07:56:06.xml |
DigitalObjectIdentifier | |
ReviewLevel | Peer-reviewed dataset |
DatasetOrigin | Original dataset |
RepositorySupport | Unsupported dataset by repository |
PrimarySubmitter | zhang yang |
SpeciesList | scientific name: Homo sapiens (Human); NCBI TaxID: 9606; |
ModificationList | No PTMs are included in the dataset |
Instrument | Q TRAP |
Dataset History
Revision | Datetime | Status | ChangeLog Entry |
0 | 2015-09-29 01:32:49 | ID requested | |
⏵ 1 | 2018-10-24 07:56:07 | announced | |
Publication List
Yue Q, Feng L, Cao B, Liu M, Zhang D, Wu W, Jiang B, Yang M, Liu X, Guo D, Proteomic Analysis Revealed the Important Role of Vimentin in Human Cervical Carcinoma HeLa Cells Treated With Gambogic Acid. Mol Cell Proteomics, 15(1):26-44(2016) [pubmed] |
Keyword List
curator keyword: Biomedical |
submitter keyword: gambogic acid, anti-cancer, proteomics, vimentin |
Contact List
De-An Guo |
contact affiliation | Shanghai Institute of Materia Medica, Chinese Academy of Sciences |
contact email | daguo@simm.ac.cn |
lab head | |
zhang yang |
contact affiliation | fudan |
contact email | zhangyang@fudan.edu.cn |
dataset submitter | |
Full Dataset Link List
Dataset FTP location
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PRIDE project URI |
Repository Record List
[ + ]
[ - ]
- PRIDE
- PXD002985
- Label: PRIDE project
- Name: Proteomic analysis revealed the important role of vimentin in human cervical carcinoma HeLa cells treated with gambogic acid